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dc.contributor.authorJosé, V. A.por
dc.contributor.authorRamos, P.por
dc.contributor.authorValcarcel, J.por
dc.contributor.authorAntelo, Luis, T.por
dc.contributor.authorNovoa-Carballal, R.por
dc.contributor.authorReis, R. L.por
dc.contributor.authorPérez Martín, R. I.por
dc.identifier.citationJosé V. A., Ramos P., Valcarcel J., Antelo Luis T., Novoa-Carballal R., Reis R. L., Pérez Martín R. I. An integral and sustainable valorisation strategy of squid pen by-products, Journal Of Cleaner Production, Vol. 201, pp. 207-218, doi:10.1016/j.jclepro.2018.07.316, 2018por
dc.description.abstractThe aim of this study was to present a sustainable valorisation approach of Loligo opalescens squid pen by-products by optimal production of chitin and chitosan biopolymers and recycling of the generated effluents. The integral recovery of squid pens started with the optimization of the deproteinization process to isolate chitin, including less hazardous enzymatic alternatives to conventional chemical treatments. Response surface methodology analysis yielded optimal conditions of 53.2 °C/pH 8.84 for Alcalase, 54.7 °C/pH 9.16 for Esperase and 0.2 M NaOH/27.5 °C for alkaline hydrolysis. The generated effluents were a good source of organic nitrogen (squid peptones) for the culture of a lactic acid bacterium (Pediococcus acidilactici), with biomass and metabolite productions comparable to commercial Man, Rogosa, Sharpe medium. Secondly, optimal process time and alkali concentration for the chemical deacetylation of chitin were established at 16 h/64% NaOH, obtaining high purity chitosan (deacetylation degrees >92%, molecular weights -Mn- ranging from 139 to 300 kDa). Optimized chitin and chitosan processes led to lower energetic costs. Alkaline effluents from chitosan production were applied as a reagent in the selective precipitation of chondroitin sulphate from enzymatic hydrolysates of shark cartilage by-products. Finally, the goodness of the proposed valorisation strategy was evaluated by developing a Material Flow Analysis.por
dc.description.sponsorshipThe authors thank to Ana Durán, Margarita Nogueira, Araceli Menduíña and Javier Fraguas for their excellent technical assistance. This research was funded by the projects IBEROS (0245_IBEROS_1_E, POCTEP 2015), CVMar+I (0302_CVMAR_I_1_P, POCTEP 2015) and BLUEHUMAN (EAPA_151/2016, UE-INTERREG Atlantic Area Programme). RNC thanks the Portuguese Foundation for Science and Technology (IF/00373/2014).por
dc.subjectsquid penspor
dc.subjectLoligo opalescens penpor
dc.subjectMarine-squid peptonespor
dc.subjectIntegral valorisationpor
dc.titleAn integral and sustainable valorisation strategy of squid pen by-productspor
dc.subject.wosScience & Technologypor
sdum.journalJournal Of Cleaner Productionpor
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