Please use this identifier to cite or link to this item:
|Title:||A novel approach for the production of human recombinant BMP-2 for bone tissue engineering applications|
|Author(s):||Bessa, Paulo C.|
Pedro, A. J.
Reis, R. L.
|Publisher:||European Society of Biochemical Engineering Science (ESBES)|
|Abstract(s):||Bone tissue engineering has been an increasing field of research during the last years. The ideal approach for a regenerative application would consist in the use of cells from the patient, scaffolding materials and differentiation growth factors. Bone morphogenetic protein-2 (BMP-2) is one such growth factors with a strong ability to induce new bone and cartilage formation and has been used as a powerful osteoinductive component of several late-stage tissue engineering products for bone grafting. In this work, we aimed at obtaining high yields of human recombinant BMP-2 in a stable, pure and biologically active form by use of a new bacteria expression system that circumvents the disadvantages of conventional recombinant protein preparation methods and to perform a study of the stability conditions and the functionality of these peptides in vitro in human mesenchymal stem cells and C2C12 murine cell line.|
|Appears in Collections:||DBio - Comunicações/Communications in Congresses|
Files in This Item:
|1180602808_Poster Salzburg ESBES2006.pdf||394,28 kB||Adobe PDF||View/Open|