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TitleA novel approach for the production of human recombinant BMP-2 for bone tissue engineering applications
Author(s)Bessa, Paulo C.
Pedro, A. J.
Nobre, Alexandra
Dungl, P.
Klosch, B.
Redl, Heinz
Casal, Margarida
Reis, R. L.
KeywordsTissue engineering
Issue date2006
PublisherEuropean Society of Biochemical Engineering Science (ESBES)
Abstract(s)Bone tissue engineering has been an increasing field of research during the last years. The ideal approach for a regenerative application would consist in the use of cells from the patient, scaffolding materials and differentiation growth factors. Bone morphogenetic protein-2 (BMP-2) is one such growth factors with a strong ability to induce new bone and cartilage formation and has been used as a powerful osteoinductive component of several late-stage tissue engineering products for bone grafting. In this work, we aimed at obtaining high yields of human recombinant BMP-2 in a stable, pure and biologically active form by use of a new bacteria expression system that circumvents the disadvantages of conventional recombinant protein preparation methods and to perform a study of the stability conditions and the functionality of these peptides in vitro in human mesenchymal stem cells and C2C12 murine cell line.
AccessOpen access
Appears in Collections:DBio - Comunicações/Communications in Congresses

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