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https://hdl.handle.net/1822/49311
Título: | An overview of the evolution of infrared spectroscopy applied to bacterial typing |
Autor(es): | Quintelas, Cristina Ferreira, Eugénio C. Lopes, João A. Sousa, C. |
Palavras-chave: | Infrared spectroscopy Bacteria Microbiology Bacterial typing chemometrics |
Data: | 2018 |
Editora: | Wiley-Blackwell |
Revista: | Biotechnology Journal |
Citação: | Quintelas, Cristina; Ferreira, Eugénio C.; Lopes, João A.; Sousa, C., An overview of the evolution of infrared spectroscopy applied to bacterial typing. Biotechnology Journal, 13(1700449), 2018 |
Resumo(s): | The sustained emergence of new declared bacterial species makes typing a continuous challenge for microbiologists. Molecular biology techniques have a very significant role in the context of bacterial typing, but they are often very laborious, time consuming and eventually fail when dealing with very closely related species. Spectroscopic-based techniques appear in some situations as a viable alternative to molecular methods with advantages in terms of analysis time and cost. Infrared and mass spectrometry are among the most exploited techniques in this context: particularly, infrared spectroscopy emerged as a very promising method with multiple reported successful applications. This article presents a systematic review on infrared spectroscopy applications for bacterial typing, highlighting fundamental aspects of infrared spectroscopy, a detailed literature review (covering different taxonomic levels and bacterial species), advantages and limitations of the technique over molecular biology methods and a comparison with other competing spectroscopic techniques such as MALDI-TOF MS, Raman and intrinsic fluorescence. Infrared spectroscopy possesses a high potential for bacterial typing at distinct taxonomic levels and worthy of further developments and systematization. The development of databases appears fundamental towards the establishment of infrared spectroscopy as a viable method for bacterial typing. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/49311 |
DOI: | 10.1002/biot.201700449 |
ISSN: | 1860-6768 |
e-ISSN: | 1860-7314 |
Versão da editora: | http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47093_1.pdf | 1,07 MB | Adobe PDF | Ver/Abrir |