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TitleYeast metabolic chassis designs for diverse biotechnological products
Author(s)Jouhten, Paula
Boruta, T.
Andrejev, S.
Pereira, Filipa
Rocha, Isabel
Patil, K. R.
Issue date19-Jul-2016
PublisherNature Publishing Group
JournalScientific Reports
CitationJouhten, Paula; Boruta, T.; Andrejev, S.; Pereira, Filipa; Rocha, Isabel; Patil, K. R., Yeast metabolic chassis designs for diverse biotechnological products. Scientific Reports, 6(29694), 1-9, 2016
Abstract(s)The diversity of industrially important molecules for which microbial production routes have been experimentally demonstrated is rapidly increasing. The development of economically viable producer cells is, however, lagging behind, as it requires substantial engineering of the host metabolism. A chassis strain suitable for production of a range of molecules is therefore highly sought after but remains elusive. Here, we propose a genome-scale metabolic modeling approach to design chassis strains of Saccharomyces cerevisiae - a widely used microbial cell factory. For a group of 29 products covering a broad range of biochemistry and applications, we identified modular metabolic engineering strategies for re-routing carbon flux towards the desired product. We find distinct product families with shared targets forming the basis for the corresponding chassis cells. The design strategies include overexpression targets that group products by similarity in precursor and cofactor requirements, as well as gene deletion strategies for growth-product coupling that lead to non-intuitive product groups. Our results reveal the extent and the nature of flux re-routing necessary for producing a diverse range of products in a widely used cell factory and provide blueprints for constructing pre-optimized chassis strains.
DescriptionSupplementary information accompanies this paper at
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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