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|Title:||Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering|
|Author(s):||Fernandes, João S.|
Martins, Margarida Isabel Barros Coelho
Neves, N. M.
Pires, R. A.
Reis, R. L.
Strontium borosilicate glasses
|Citation:||Fernandes J. S., Gentile P., Martins I. M., Neves N. M., Miller C., Crawford A., Pires R. A., Hatton P. V., Reis R. L. Reinforcement of Poly-L-lactic acid Electrospun Membranes with Strontium Borosilicate Bioactive Glasses for Bone Tissue Engineering, Acta Biomaterialia, Vol. 44, pp. 168-177, doi:10.1016/j.actbio.2016.08.042, 2016|
|Abstract(s):||Herein, for the first time, we combined poly-L-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass par- ticles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (Î¼-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1â 3 Î¼m in width) with a homogeneous distribution of BBG-Sr microparticles (<45 Î¼m). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membraneâ s mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr mem- branes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomater- ial capable of promoting bone regeneration.Â|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|