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https://hdl.handle.net/1822/40286
Título: | Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: a surface-roughness gradient study |
Autor(es): | Torres, Ana B. Faia Charnley, Mirren Goren, Tolga Lischer, Stefanie Guimond Rottmar, Markus Weber, Katharina Maniura Spencer, Nicholas D. Reis, R. L. Textor, Marcus Neves, N. M. |
Palavras-chave: | Biomaterials High-throughput screening (gradient) Microstructures Mesenchymal stem cells Osteogenesis |
Data: | Out-2015 |
Editora: | Elsevier |
Revista: | Acta Biomaterialia |
Citação: | Faia-Torres A. B., Charnley M., Goren T., Guimond-Lischer S., Rottmar M., Maniura-Weber K., Spencer N. D., Reis R. L., Textor M., Neves N. M. Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: a surface-roughness gradient study., Acta Biomaterialia, Vol. 28, pp. 64-75, doi:10.1016/j.actbio.2015.09.028, 2015 |
Resumo(s): | The use of biomaterials to direct osteogenic differentiation of human mesenchymal stem cells (hMSCs) in the absence of osteogenic supplements is thought to be part of the next generation of orthopedic implants. We previously engineered surface-roughness gradients of average roughness (Ra) varying from the sub-micron to the micrometer range ( 0.5–4.7 lm), and mean distance between peaks (RSm) gradually varying from 214 lm to 33 lm. Here we have screened the ability of such surface-gradients of polycaprolactone to influence the expression of alkaline phosphatase (ALP), collagen type 1 (COL1) and mineralization by hMSCs cultured in dexamethasone (Dex)-deprived osteogenic induction medium (OIM) and in basal growth medium (BGM). Ra 1.53 lm/RSm 79 lm in Dex-deprived OI medium, and Ra 0.93 lm/RSm 135 lm in BGM consistently showed higher effectiveness at supporting the expression of the osteogenic markers ALP, COL1 and mineralization, compared to the tissue culture polystyrene (TCP) control in complete OIM. The superior effectiveness of specific surface-roughness revealed that this strategy may be used as a compelling alternative to soluble osteogenic inducers in orthopedic applications featuring the clinically relevant biodegradable polymer polycaprolactone. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/40286 |
DOI: | 10.1016/j.actbio.2015.09.028 |
ISSN: | 1742-7061 |
Versão da editora: | http://www.sciencedirect.com/science/article/pii/S1742706115301215 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18628-Torres_ActaBio 2015_ahead of print.pdf Acesso restrito! | 5,81 MB | Adobe PDF | Ver/Abrir |