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https://hdl.handle.net/1822/39315
Título: | Poly-N-acetyl glucosamine expression in Staphylococcus epidermidis biofilm cells affects the immune response and promotes tissue pathology in infected hosts |
Autor(es): | Ferreirinha, Pedro Pérez-Cabezas, Begoña Correia, Alexandra Miyazawa, Bruna Carvalhais, Virgínia Maria Dinis França, Ângela Maria Oliveira Sousa Faustinho, Augusto Cordeiro-da-Silva, Anabela Teixeira, Luzia Pier, Geral Cerca, Nuno Vilanova, Manuel |
Data: | 22-Set-2015 |
Citação: | Ferreirinha, P.; Pérez-Cabezas, B.; Correia, A.; Miyazawa, B.; Carvalhais, V.; França, Ângela; Faustinho, A.; Cordeiro-da-Silva, A.; Teixeira, L.; Pier, G. B.; Cerca N; Vilanova, M., Poly-N-acetylglucosamine expression in Staphyloccocus epidermidis biofilm cells affects the imune response and promotes tissue pathology in infected hosts. XXXVI Annual Meeting of the Portuguese Society for Immunology. Braga, Portugal, 22-24 September, 2015. |
Resumo(s): | Staphylococcus epidermidis is a biofilm - forming bacterium and a leading etiological agent of nosocomial infections. The ability to establish biofilms on indwelling medical devices is a key virulence factor for this bacterium. Still, the influence of poly - N - acetyl glucosamine (PNAG), the major component of the extracellular biofilm matrix, in the host immune response has been scarcely studied. Here, t h is influence was assessed in mice challenged i.p. with PNAG - p roducing (WT) and isogenic - mutant lacking PNAG (M10) bacteria grown in biofilm - inducing conditions. Faster bacterial clearance was observed in the mice infected with WT bacteria than in M10 - infected counterparts , which w as accompanied by earlier neutrophil recruitment and higher IL - 6 production. Interestingly, in the WT - infected mice, but not in those infected with M10 , elevated serum IL - 10 was detected . To further study the effe ct of PNAG in the immune response, mice were primed with WT or M10 biofilm bacteria and subsequently infected with WT biofilm - released cells. WT - primed mice presented a higher frequency of splenic IFN - γ + and IL - 17 + CD4 + T cells, and more severe liver patho logy than M10 - primed counterparts. Nevertheless, T reg cells obtained from the WT - primed mice presented a higher suppressive function than those obtained from M10 - primed mice. This effect was abrogated when IL - 10 - deficient mice were similarly primed and infected indicating that PNAG promotes the differentiati on of highly suppressive T reg cells by a mechanism dependent on IL - 10. Altogether, these results provide evidence help ing explain ing the coexistence of inflammation and bacterial persistence often observed in biofilm - originated S. epidermidis infections |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/39315 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
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