Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/34586

TitleAn immunoproteomic approach for characterization of dormancy within Staphylococcus epidermidis biofilms
Author(s)Carvalhais, Virgínia Maria Dinis
Cerveira, Frederico
Vilanova, Manuel
Cerca, Nuno
Vitorino, Rui
KeywordsS. epidermidis biofilm
Dormancy
Immunoproteomics
Issue dateJun-2015
PublisherElsevier
JournalMolecular immunology
CitationCarvalhais, V.; Cerveira, F.; Vilanova, M.; Cerca, Nuno; Vitorino, R., An immunoproteomic approach for characterization of dormancy within Staphylococcus epidermidis biofilms. Molecular Immunology, 65(2), 429-435, 2015
Abstract(s)Virulence of Staphylococcus epidermidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. Physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. Dormant cells, among others, contribute to biofilm heterogeneity. The aim of this study was to identify immunogenic proteins of S. epidermidis biofilms associated with dormancy mechanism, by using two-dimensional electrophoresis (2-DE) immunoblotting and mass spectrometry (MS). A total of 19 bacterial proteins, recognized by human serum samples, were identified. These proteins were mainly involved in small molecule metabolic biological processes. Catalytic activity and ion binding were the most representative molecular functions. CodY and GpmA proteins were more reactive to sera when biofilm dormancy was induced, while FtnA and ClpP were more reactive when dormancy was prevented. This is the first work that identifies differences in immunoreactive proteins within bacterial biofilms with induced or prevented dormancy. Considering the importance of dormancy within biofilms, further evaluation of these proteins can provide insights into the mechanisms related to dormancy and help to improve current understanding on how dormancy affects the host immune response.
TypeArticle
URIhttp://hdl.handle.net/1822/34586
DOI10.1016/j.molimm.2015.02.024
ISSN0161-5890
e-ISSN0161-5890
Publisher versionhttp://www.elsevier.com/locate/molimm
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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