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TitleBioconversion of agro-industrial by-products in rhamnolipids toward applications in enhanced oil recovery and bioremediation
Author(s)Gudiña, Eduardo J.
Rodrigues, A. I.
Alves, Eliana
Domingues, M. R. M.
Teixeira, J. A.
Rodrigues, L. R.
Pseudomonas aeruginosa
Corn step liquor
Enhanced oil recovery
Corn steep liquor
Issue dateFeb-2015
PublisherElsevier B.V.
JournalBioresource Technology
CitationGudiña, Eduardo J.; Rodrigues, A. I.; Alves, Eliana; Domingues, M. Rosário; Teixeira, J. A.; Rodrigues, L. R., Bioconversion of agro-industrial by-products in rhamnolipids towards applications in enhanced oil recovery and bioremediation. Bioresource Technology, 177, 87-93, 2015
Abstract(s)In this work, biosurfactant production by a Pseudomonas aeruginosa strain was optimized using low-cost substrates. The highest biosurfactant production (3.2 g/l) was obtained using a culture medium containing corn step liquor (10% (v/v)) and molasses (10% (w/v)). The biosurfactant reduced the surface tension of water up to 30 mN/m, and exhibited a high emulsifying activity (E24= 60%), with a critical micelle concentration as low as 50 mg/l. The biosurfactant produced in this alternative medium was characterized as a mixture of eight different rhamnolipid congeners, being the most abundant the mono-rhamnolipid Rha-C10-C10. However, using LB medium, nine different rhamnolipid congeners were identified, being the most abundant the di-rhamnolipid Rha-Rha-C10-C10. The rhamnolipid mixture produced in the alternative medium exhibited a better performance in removing oil from contaminated sand when compared with two chemical surfactants, suggesting its potential use as an alternative to traditional chemical surfactants in enhanced oil recovery or bioremediation.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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