Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/32515

TitleComparative genomics of Lactobacillus crispatus suggests novel mechanisms for the competitive exclusion of Gardnerella vaginalis
Author(s)Ojala, Teija
Kankainen, Matti
Castro, J.
Cerca, Nuno
Edelman, Sanna
Westerlund-Wikström, Benita
Paulin, Lars
Holm, Liisa
Auvinen, Petri
KeywordsBacterial vaginosis
Comparative genomics
Competitive exclusion
Core genome
Gardnerella vaginalis
Lactobacillus crispatus
Normal flora
Pan-genome
Issue date2014
PublisherBioMed Central (BMC)
JournalBMC Genomics
CitationOjala, Teija; Kankainen, Matti; Castro, Joana; Cerca, Nuno; Edelman, Sanna; Westerlund-Wikström, Benita; Paulin, Lars; Holm, Liisa; Auvinen, Petri, Comparative genomics of Lactobacillus crispatus suggests novel mechanisms for the competitive exclusion of Gardnerella vaginalis. BMC Genomics, 15(1070), 2014
Abstract(s)Background: Lactobacillus crispatus is a ubiquitous micro-organism encountered in a wide range of host-associated habitats. It can be recovered from the gastrointestinal tract of animals and it is a common constituent of the vaginal microbiota of humans. Moreover, L. crispatus can contribute to the urogenital health of the host through competitive exclusion and the production of antimicrobial agents. In order to investigate the genetic diversity of this important urogenital species, we performed a comparative genomic analysis of L. crispatus. Results: Utilizing the completed genome sequence of a strain ST1 and the draft genome sequences of nine other L. crispatus isolates, we defined the scale and scope of the pan- and core genomic potential of L. crispatus. Our comparative analysis identified 1,224 and 2,705 ortholog groups present in all or only some of the ten trains, respectively. Based on mathematical modeling, sequencing of additional L. crispatus isolates would result in the identification of new genes and functions, whereas the conserved core of the ten strains was a good representation of the final L. crispatus core genome, estimated to level at about 1,116 ortholog groups. Importantly, the current core was observed to encode bacterial components potentially promoting urogenital health. Using antibody fragments specific for one of the conserved L. crispatus adhesins, we demonstrated that the L. crispatus core proteins have a potential to reduce the ability of Gardnerella. vaginalis to adhere to epithelial cells. These findings thereby suggest that L. crispatus core proteins could protect the vagina from G. vaginalis and bacterial vaginosis. Conclusions Our pan-genome analysis provides insights into the intraspecific genome variability and the collective molecular mechanisms of the species L. crispatus. Using this approach, we described the differences and similarities between the genomes and identified features likely to be important for urogenital health. Notably, the conserved genetic backbone of L. crispatus accounted for close to 60% of the ortholog groups of an average L. crispatus strain and included factors for the competitive exclusion of G. vaginalis, providing an explanation on how this urogenital species could improve vaginal health.
TypeArticle
URIhttp://hdl.handle.net/1822/32515
DOI10.1186/1471-2164-15-1070
ISSN1471-2164
Publisher versionhttp://www.biomedcentral.com/1471-2164/15/1070
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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