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TitleGa[NO2A-N-(alfa-amino)propionate] chelates: synthesis and evaluation as potential tracers for 68Ga3+ PET
Author(s)Ferreira, Miguel F.
Pereira, Goreti
André, João P.
Prata, M. I. M.
Ferreira, Paula M. T.
Martins, José A.
Geraldes, Carlos F. G. C.
KeywordsNO2A-N-(alfa-amino)propionic acid chelators
Ga3+ complexes
1H and 71Ga NMR
67Ga3+-labelled chelates
Serum stability
Issue date9-Apr-2014
PublisherRoyal Society of Chemistry
JournalDalton Transactions
CitationFerreira, M. F.; Pereira, G.; André, J. P.; Prata, M. I. M.; Ferreira, P. M. T.; Martins, J. A.; Geraldes, C. F. G. C. Ga[NO2A-N-(α-amino)propionate] chelates: synthesis and evaluation as potential tracers for 68Ga PET. Dalton Trans., 2014, 43, 8037-8047. DOI: 10.1039/C4DT00386A
Abstract(s)The availability of commercial 68Ge/68Ga cyclotron-independent 68Ga3+ generators is making Positron Emission Tomography (PET) accessible to most hospitals, which is generating a surge of interest in the design and synthesis of bi-functional chelators for Ga3+. In this work we introduce the NO2A-N-(alfa-amino)propionic acid family of chelators based on the triazacyclononane scaffold. Complexation of the parent NO2A-N-(alfa-amino)propionic acid chelator and of a low molecular weight (model) amide conjugate with Ga3+ was studied by 1H and 71Ga NMR. The Ga3+ chelate of the amide conjugate shows pH-independent N3O3 coordination in the pH range 3-10 involving the carboxylate group of the pendant propionate arm in a 6 member chelate. For the Ga[NO2A-N-(alfa-amino)propionate] chelate, a reversible pH-triggered switch from Ga3+ coordination to the carboxylate group to coordination to the amine group of the propionate arm, was observed upon pH increase/decrease in the pH range 4-6. This phenomenon can conceivably constitute the basis of a physiological pH sensor. Both complexes are stable in the physiological range. The [67Ga][NO2A-N-(alfa-benzoylamido)propionate] chelate was found to be stable in human serum. Biodistribution studies of the 67Ga3+-labeled pyrene butyric acid conjugate NO2A-N-(alfa-pyrenebutanamido)propionic acid revealed that, despite its high lipophilicity and concentration-dependent aggregation properties, the chelate follows mainly renal elimination with very low liver/spleen accumulation and no activity deposition in bones after 24 hours. Facile synthesis of amide conjugates of the NO2A-N-(alfa-amino)propionic acid chelator, serum stability of the Ga3+chelates and fast renal elimination warrant further evaluation of this novel class of chelators for PET applications.
AccessOpen access
Appears in Collections:CDQuim - Artigos (Papers)

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