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https://hdl.handle.net/1822/30983
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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, Joana M. | por |
dc.contributor.author | Duarte, Ana Rita C. | por |
dc.contributor.author | Caridade, S. G. | por |
dc.contributor.author | Picart, Catherine | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Mano, J. F. | por |
dc.date.accessioned | 2014-11-18T16:13:26Z | - |
dc.date.available | 2014-11-18T16:13:26Z | - |
dc.date.issued | 2014-09 | - |
dc.date.submitted | 2014-11 | - |
dc.identifier.issn | 1525-7797 | por |
dc.identifier.uri | https://hdl.handle.net/1822/30983 | - |
dc.description.abstract | Engineering metabolically demanding tissues requires the supply of nutrients, oxygen, and removal of metabolic byproducts, as well as adequate mechanical properties. In this work, we propose the development of chitosan (CHIT)/alginate (ALG) freestanding membranes fabricated by layer-by-layer (LbL) assembly. CHIT/ALG membranes were cross-linked with genipin at a concentration of 1 mg· mL−1 or 5 mg·mL−1. Mass transport properties of glucose and oxygen were evaluated on the freestanding membranes. The diffusion of glucose and oxygen decreases with increasing cross-linking concentration. Mechanical properties were also evaluated in physiological-simulated conditions. Increasing cross-linking density leads to an increase of storage modulus, Young modulus, and ultimate tensile strength, but to a decrease in the maximum hydrostatic pressure. The in vitro biological performance demonstrates that cross-linked films are more favorable for cell adhesion. This work demonstrates the versatility and feasibility of LbL assembly to generate nanostructured constructs with tunable permeability, mechanical, and biological properties. | por |
dc.description.sponsorship | The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through the doctoral and Postdoctoral grants with the reference numbers SFRH/BD/81372/2011 (JMS) and SFRH/BPD/96797/2013 (SGC), respectively. This work was financially supported by the FCT, by the project PTDC/FIS/115048/2009, and by the European Commission/FP7 programme (ERC Starting Grant, GA 259370 to C.P.). The authors would also like to acknowledge the project novel smart and biomimetic materials for innovative regenerative medicine approaches (ref.: RL1 - ABMR - NORTE-01-0124-FEDER-000016) cofinanced by the North Portugal Regional Operational Programme (ON.2 0 Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF). | por |
dc.language.iso | eng | por |
dc.publisher | ACS Publications | por |
dc.rights | restrictedAccess | por |
dc.subject | Alginate | por |
dc.subject | Chitosan | por |
dc.subject | Diffusion | por |
dc.title | Tailored freestanding multilayered membranes based on chitosan and alginate | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://pubs.acs.org/doi/abs/10.1021/bm501156v | por |
dc.comments | http://www.3bs.uminho.pt/node/18165 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 3817 | por |
oaire.citationEndPage | 3826 | por |
oaire.citationIssue | 10 | por |
oaire.citationTitle | Biomacromolecules | por |
oaire.citationVolume | 15 | por |
dc.date.updated | 2014-11-11T15:12:11Z | - |
dc.identifier.doi | 10.1021/bm501156v | - |
dc.identifier.pmid | 25244323 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomacromolecules | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18165-bm501156v.pdf Acesso restrito! | 7,42 MB | Adobe PDF | Ver/Abrir |