Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24962

TitleDual effect of platelet lysate on human articular cartilage : a maintenance of chondrogenic potential and a transient pro-inflammatory activity followed by an inflammation resolution
Author(s)Pereira, R. C.
Scaranari, M.
Benelli, R.
Strada, P.
Reis, R. L.
Cancedda, Ranieri
Gentili, Chiara
KeywordsHuman articular chondrocytes
Inflammation
Issue dateApr-2013
PublisherMary Ann Liebert
JournalTissue Engineering : part A
Abstract(s)Platelet-rich plasma (PRP), a cocktail of platelet growth factors and bioactive proteins, has been proposed as a therapeutic agent to restore damaged articular cartilage. We report the biological effect of the platelet lysate (PL), a PRP derivative, on primary human articular chondrocytes cultured under both physiological and inflammatory conditions. When added to the culture medium, PL induced a strong mitogenic response in the chondrocytes. The in vitro expanded cell population maintained a chondrogenic redifferentiation potential as revealed by micromass culture in vitro and ectopic cartilage formation in vivo. Further, in chondrocytes cultured in the presence of the proinflammatory cytokine interleukin-1a (IL-1a), the PL induced a drastic enhancement of the synthesis of the cytokines IL-6 and IL-8 and of neutrophil-gelatinase associated lipocalin, a lipocalin expressed during chondrocyte differentiation and inflammation. These events were mediated by the p38 MAP kinase and NF-kB pathways. We observed that inflammatory stimuli activated phospo-MAP kinase-activated protein kinase 2, a direct target of p38. The proinflammatory effect of the PL was a transient phenomenon; after an initial upregulation, we observed significant reduction of the NF-kB activity together with the repression of the inflammatory enzyme cyclooxygenase-2. Moreover, the medium of chondrocytes cultured in the simultaneous presence of PL and IL-1a, showed a significant enhancement of the chemoattractant activity versus untreated chondrocytes. Our findings support the concept that the platelet products have a direct beneficial effect on articular chondrocytes and could drive in sequence a transient activation and the resolution of the inflammatory process, thus providing a rational for their use as therapeutic agents in cartilage inflammation and damage.
TypeArticle
URIhttp://hdl.handle.net/1822/24962
DOI10.1089/ten.tea.2012.0225
ISSN2152-4947
Publisher versionhttp://online.liebertpub.com/doi/abs/10.1089/ten.TEA.2012.0225
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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