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TitleDevelopment of macro/micro porous silk fibroin scaffolds with nano-sized calcium phosphate particles for bone tissue engineering
Author(s)Yan, Leping
Correia, Cristina
Silva-Correia, Joana
Caridade, S. G.
Fernandes, E. M.
Mano, J. F.
Sousa, R. A.
Oliveira, Joaquim M.
Oliveira, A. L.
Reis, R. L.
Silk fibroin
Issue dateSep-2012
PublisherJohn Wiley and Sons
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationYan L. - P. Yan L. P., Correia C., Silva-Correia J., Caridade S. G., Fernandes E. M., Mano J. F., Sousa R. A., Oliveira J. M., Oliveira A. L., Reis R. L. Preparation and characterization of macro/micro porous silk fibroin /nano-sized calcium phosphate scaffolds for bone tissue engineering, Journal of tissue engineering and regenerative medicine, Vol. 6, Issue Suppl.1, pp. 181, doi:10.1002/term.1586, 2012
Abstract(s)Macro/micro porous silk scaffolds with nano-sized calcium phosphate (CaP) particles were developed for bone tissue engineering. Different amounts of nano-sized CaP particles 4, 8, 16 and 25% (CaP:silk fibroin, wt/wt) were generated into the highly concentrated aqueous silk fibroin solutions via an in-situ approach. Afterwards, the pure silk and silk/nano-CaP porous scaffolds were produced by a combination of salt-leaching/freeze-drying methods. Thermal gravimetric analysis results were able to demonstrated that the silk/nano-CaP scaffolds maintained 64–87% of incorporated CaP after salt-leaching. Dynamic mechanical analysis showed that storage modulus of the 16% formulation was significantly higher than all remaining groups. The porosity of silk/nano-CaP scaffolds assessed using Micro-Computed Tomography decreased from 79.8% to 63.6% with increasing CaP incorporated until 16%. By soaking the scaffolds in Simulated Body Fluid for 7 days, cauliflower-like apatite clusters were observed on the surface of both macro and micro pores of 16% and 25% formulations, which was not observed in 4 and 8% formulations. 16% silk/nano-CaP scaffolds were further chosen for in vitro cytotoxicity and biocompatibility assays. Both silk and 16% silk/nano-CaP scaffolds were non-cytotoxic and promoted cell adhesion and proliferation to a similar extent. These results indicated that the 16% silk/ nano-CaP scaffolds could be a good candidate for bone tissue engineering.
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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