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https://hdl.handle.net/1822/23863
Título: | Pericytes contribute to the stability of the vascular network of osteogenic tissue formed from cell sheet-based constructs |
Autor(es): | Mendes, L. F. Pirraco, Rogério P. Szymczyk, Wojciech Santos, T. C. Frias, A. M. Reis, R. L. Marques, A. P. |
Palavras-chave: | Cell sheets Endothelial cells Osteoblasts Pericytes |
Data: | Set-2012 |
Editora: | John Wiley and Sons |
Revista: | Journal of Tissue Engineering and Regenerative Medicine |
Resumo(s): | Despite the increased use of cell sheets for tissue engineering, the appli- cation of this new technology for thick tissue reconstruction is being limited by old barriers such as the need to create a pre-vascular net- work to ensure proper nutrient and oxygen supply in vivo. In the pres- ent work, we created a cell sheet-based construct by co-culturing bone marrow-derived osteogenic and pericyte-like cells with human umbili- cal vein endothelial cells (HUVECs) with the purpose of enhancing the in vivo vascularization of newly formed osteogenic tissue. Human bone marrow mesenchymal stromal cells (hBMSCs) were isolated and cul- tured in medium supplemented with osteogenic factors or TGF-b1 to obtain either osteogenic or CD146+ pericyte-like cells. Immunocyto- chemistry analysis of the co-cultures showed organized structures formed by CD146+ hBMSCs and HUVECs over the osteogenic cell sheet, suggesting the existence of cross-talk between the co-cultured cell types. Nude mice were used to test the ability of those constructs to form functional and vascularized osteogenic tissue. Immunohisto- chemistry analysis of cell sheet-based constructs after 1 and 3 weeks of transplantation revealed the integration of HUVECs with neighboring host’s vessels. Additionally, the analysis of the diameter of the blood vessels showed a higher mean diameter for the condition that com- bined pericyte-like cells and HUVECs, reinforcing the advantage of the proposed model regarding blood vessels maturation and stability. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/23863 |
ISSN: | 1932-6254 |
Versão da editora: | http://onlinelibrary.wiley.com/doi/10.1002/term.1586/abstract |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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17328-TERMIS2012_RPPirraco.pdf | 292,95 kB | Adobe PDF | Ver/Abrir |