Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/22630
Título: | Protein disulphide isomerase-induced refolding of sonochemically prepared Ribonuclease A microspheres |
Autor(es): | Fernandes, Margarida M. Silva, Raquel Ferreira, Helena Donelli, Ilaria Freddi, G. Paulo, Artur Cavaco |
Palavras-chave: | Protein disulphide isomerase Ribonuclease A Ultrasound Microspheres Activity recovery Protein release Protein folding |
Data: | 2012 |
Editora: | Elsevier 1 |
Revista: | Journal of Biotechnology |
Resumo(s): | The present communication describes for the first time the development of Ribonuclease A (RNase A) microspheres using the sonochemical method followed by an enzymatic treatment with protein disulphide isomerase (PDI). Ultrasound application induced changes on the protein physicochemical and biological properties: the enzymatic activity of RNase A was decreased in 35% and the free thiol groups content was significantly increased, probably due to the breakage of protein disulphide bonds and assembly of RNase A monomers. The deconvolution of amide I band, from Fourier Transform Infrared Spectroscopy, showed that the secondary structure of RNase A was slightly changed after microspherization. The PDI application on microspheres promoted the recovery of RNase A biological activity and induced the release of active protein into solution in its native state. These results were promoted by different states of PDI active site: oxidized and reduced, respectively. The PDI aptitude to catalyze the refolding of a protein substrate in the form of spheres is here reported. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/22630 |
DOI: | 10.1016/j.jbiotec.2012.02.016 |
ISSN: | 0168-1656 |
Versão da editora: | 10.1016/j.jbiotec.2012.02.016 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2012 Protein disulphide isomerase-induced refolding of sonochemically prepared Ribonuclease A microspheres.pdf | Protein disulphide isomerase-induced refolding of sonochemically prepared Ribonuclease A microspheres | 700,29 kB | Adobe PDF | Ver/Abrir |