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https://hdl.handle.net/1822/1575| Título: | Exo and endo-glucanolytic activity of cellulases purified from Trichoderma reesei |
| Autor(es): | Gama, F. M. Vilanova, Manuel Mota, M. |
| Data: | Set-1998 |
| Editora: | Chapman & Hall |
| Revista: | Biotechnology Techniques |
| Citação: | "Biotechnology techniques". ISSN 0951-208X. 12:9 (1998) 677-681. |
| Resumo(s): | Four cellulases, produced by Trichoderma reesei, have been purified by preparative isoelectric focusing (Rotofor), size exclusion (Sephacryl 100 HR), anionic (Mono Q) and cationic (Mono S) chromatography and chromatofocusing (Mono P). Enzymatic activity with a large number of substrates allowed the proteins to be classified as: cellobiohydrolase I, cellobiohydrolase II, endoglucanase I and endoglucanase II. The exo- or endo- glucanase character of these enzymes was analysed by using a technique based on the measurement of the Avicel insoluble fibres reducing power. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/1575 |
| DOI: | 10.1023/A:1008808803109 |
| ISSN: | 0951-208X |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| 1998-14[1].pdf | 284,73 kB | Adobe PDF | Ver/Abrir |
