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TitleBehavior of single and mixed biofilms subjected to benzalkonium chloride : the impact of using chemical-adapted bacteria
Author(s)Lopes, Susana Patrícia
Machado, Idalina
Vieira, M. J.
Pereira, Maria Olívia
Issue date2-Sep-2009
CitationEUROPEAN CONGRESS ON MICROBIAL BIOFILMS, 1, Rome, Italy, 2009 – “Eurobiofilms 2009 : book of abstracts”. [S.l. : s.n., 2009].
Abstract(s)The majority of lab‐scale studies involves a single species to form biofilms and to later evaluate the effect of the antimicrobials against these biofilms. However, in real systems, biofilms can be found as a complex consortium of species where the biofilm behaviour is the sum of the synergistic and antagonistic interactions between the different species. It is not surprising that disinfection protocols developed with a single species biofilm can fail when applied to a real system. The central query of this study was to understand the behaviour of binary biofilms when exposed to high doses of benzalkonium chloride (BZK), a general disinfectant in health care facilities. P. aeruginosa (ATCC 10145), P. aeruginosa adapted to 4.0 mM of BZK (PA4) and Escherichia coli k12 were the strains used to form single and binary biofilms. The mixed‐species biofilms were obtained by a combination of 50% of suspended inocula of each specie: E. coli with P. aeruginosa and E. coli with PA4. The role of surface preconditioning with BZK on biofilm properties was also investigated. Biofilms where formed for 24 h in 96 plates (non‐ and conditioned with 0.7 mM of BZK for 30 min), being then treated with 1.0 mM of BZK. Crystal violet (CV) together with XTT, were used to assess, respectively, total attached biomass and respiratory activity. The number of CFU/cm2 was also determined. The data emphasised that the characteristics of the binary biofilms depend on the strains that formed them. In fact, when the adapted strain is involved, the resulting biofilms have less mass, activity and cells. It is also observed that, for both single‐ and dual‐species biofilms, surface preconditioning significantly favoured biofilm formation. Although the BZK preconditioning of the surfaces increased biomass, it simultaneous appeared to weak bacterial adhesion, since BZK attack reduced significantly the amount of biofilm comparing to the clear surfaces, for both single‐ and mixed‐species. Concerning the data related with BZK attack, for both non‐ and conditioned surfaces, there was a notorious reduction of the bacterial activity and number of cells, in both single and mixed biofilms. But, the PA4 and E. coli binary biofilms seemed to reveal recalcitrant behaviour since the CFU still unaltered after BZK attack. These data suggest that when biofims are formed by the PA4 and E. coli, it seems to exist an competitive behaviour between the bacteria that give rise to a mixed biofilm with fewer mass and cells. However, these biofilms are less susceptible to the action of BZK, sign of a possible protective effect of one species to another. Since few reports specifically address interactions within multi‐species biofilms, these results highlight the importance of understanding the multiple behaviours of biofilms as a community and a complex web of interactions.
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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