Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/9094

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dc.contributor.authorEscamilla Garcia, Erandi-
dc.contributor.authorAguedo, Mário-
dc.contributor.authorGomes, Nelma-
dc.contributor.authorChoquet, Armelle-
dc.contributor.authorBelo, Isabel-
dc.contributor.authorTeixeira, J. A.-
dc.contributor.authorBelin, Jean-Marc-
dc.contributor.authorWaché, Yves-
dc.date.accessioned2009-05-12T14:06:21Z-
dc.date.available2009-05-12T14:06:21Z-
dc.date.issued2009-05-
dc.identifier.citation"Journal of Molecular Catalysis B : Enzymatic". ISSN 1381-1177. 57:1-4 (May 2009) 22-26.en
dc.identifier.issn1381-1177en
dc.identifier.urihttp://hdl.handle.net/1822/9094-
dc.description.abstract3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia lipolytica was placed in different environmental conditions aiming at altering β-oxidation fluxes. The concentration of substrate, pH, aeration and dissolved oxygen level were modified. We observed an important accumulation at low aeration (0.40 molar yields) and, to a lesser extent, at lower pH (0.15). As oxygen played a key-role, we evaluated its effect at fixed dissolved oxygen and at the pH which was the most favourable to the biotransformation (pH 4.5). At 5% and 30% dissolved oxygen, yields reached 0.50. β-Oxidation fluxes are very dependent on the presence of oxygen and conditions of accumulation of 3-hydroxy-γ-decalactone with very high yields were identified. These results are an important step in the production of the two decenolides. Moreover, they show the high dependence of β-oxidation fluxes on environmental conditions and relate these conditions to the accumulation of intermediates, results that are of interest to all the processes using yeast on lipids or alkanes.en
dc.description.sponsorshipRégion Bourgogne, the Programme of the French Agency for Research ANR “Transaronat” and the Programme d’Actions Universitaires Intégrées Luso- Françaises (PAUILF-CRUP); Consejo Nacional de Ciencia y Tecnología (Conacyt-Mexico); IBB-Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho.en
dc.language.isoengen
dc.publisherElsevier B.V.en
dc.rightsopenAccessen
dc.subject3-Hydroxy-γ-decalactoneen
dc.subjectβ-Oxidation fluxesen
dc.subjectOxygenen
dc.subjectYarrowia lipolyticaen
dc.subject3-Hydroxy-gamma-decalactonepor
dc.subjectbeta-Oxidation fluxespor
dc.titleProduction of 3-hydroxy-γ-decalactone, the precursor of two decenolides with flavouring properties, by the yeast Yarrowia lipolyticaen
dc.typearticlepor
dc.peerreviewedyesen
sdum.number1-4en
sdum.pagination22-26en
sdum.publicationstatuspublisheden
sdum.volume57en
oaire.citationStartPage22por
oaire.citationEndPage26por
oaire.citationIssue1-4por
oaire.citationVolume57por
dc.identifier.doi10.1016/j.molcatb.2008.06.010por
dc.subject.wosScience & Technologypor
sdum.journalJournal of Molecular Catalysis B: Enzymaticpor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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