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TitleProduction of 3-hydroxy-γ-decalactone, the precursor of two decenolides with flavouring properties, by the yeast Yarrowia lipolytica
Author(s)Escamilla Garcia, Erandi
Aguedo, Mário
Gomes, Nelma
Choquet, Armelle
Belo, Isabel
Teixeira, J. A.
Belin, Jean-Marc
Waché, Yves
β-Oxidation fluxes
Yarrowia lipolytica
beta-Oxidation fluxes
Issue dateMay-2009
PublisherElsevier B.V.
JournalJournal of Molecular Catalysis B: Enzymatic
Citation"Journal of Molecular Catalysis B : Enzymatic". ISSN 1381-1177. 57:1-4 (May 2009) 22-26.
Abstract(s)3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia lipolytica was placed in different environmental conditions aiming at altering β-oxidation fluxes. The concentration of substrate, pH, aeration and dissolved oxygen level were modified. We observed an important accumulation at low aeration (0.40 molar yields) and, to a lesser extent, at lower pH (0.15). As oxygen played a key-role, we evaluated its effect at fixed dissolved oxygen and at the pH which was the most favourable to the biotransformation (pH 4.5). At 5% and 30% dissolved oxygen, yields reached 0.50. β-Oxidation fluxes are very dependent on the presence of oxygen and conditions of accumulation of 3-hydroxy-γ-decalactone with very high yields were identified. These results are an important step in the production of the two decenolides. Moreover, they show the high dependence of β-oxidation fluxes on environmental conditions and relate these conditions to the accumulation of intermediates, results that are of interest to all the processes using yeast on lipids or alkanes.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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