Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/88831
Título: | Rice husk, brewer’s spent grain, and vine shoot trimmings as raw materials for sustainable enzyme production |
Autor(es): | Guimarães, Ana Cristina Silva Esperança Mota, Ana C. Pereira, Ana Sofia Silva Fernandes, Margarida Lopes, Marlene Belo, Isabel |
Palavras-chave: | Agro-industrial by-products Aspergillus niger Lignocellulolytic enzymes Simplex centroid mixture design Solid-state fermentation |
Data: | 2024 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Materials |
Citação: | Guimarães, Ana C.; Mota, Ana; Pereira, Ana S.; Fernandes, Margarida; Lopes, Marlene; Belo, Isabel, Rice husk, brewer's spent grain, and vine shoot trimmings as raw materials for sustainable enzyme production. Materials, 17(4), 935, 2024 |
Resumo(s): | Solid by-products with lignocellulosic structures are considered appropriate substrates for solid-state fermentation (SSF) to produce enzymes with diverse industrial applications. In this work, brewer’s spent grain (BSG), rice husk (RH), and vine shoot trimmings (VSTs) were employed as substrates in SSF with Aspergillus niger CECT 2088 to produce cellulases, xylanases, and amylases. The addition of 2% (NH4)2SO4 and 1% K2HPO4 to by-products had a positive effect on enzyme production. Substrate particle size influenced enzyme activity and the overall highest activities were achieved at the largest particle size (10 mm) of BSG and RH and a size of 4 mm for VSTs. Optimal substrate composition was predicted using a simplex centroid mixture design. The highest activities were obtained using 100% BSG for β-glucosidase (363 U/g) and endo-1,4-β-glucanase (189 U/g), 87% BSG and 13% RH for xylanase (627 U/g), and 72% BSG and 28% RH for amylase (263 U/g). Besides the optimal values found, mixtures of BSG with RH or VSTs proved to be alternative substrates to BSG alone. These findings demonstrate that SSF bioprocessing of BSG individually or in mixtures with RH and VSTs is an efficient and sustainable strategy to produce enzymes of significant industrial interest within the circular economy guidelines. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/88831 |
DOI: | 10.3390/ma17040935 |
ISSN: | 1996-1944 |
Versão da editora: | https://www.mdpi.com/1996-1944/17/4/935 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56601_1.pdf | 2,5 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons