Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/8820

TitleGalactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles
Author(s)Neri, D. F. M.
Balcão, V. M.
Dourado, Fernando
Oliveira, J. M.
Carvalho Junior, L. B.
Teixeira, J. A.
Keywordsβ-galactosidase
Magnetic support
Polysiloxane
Polyaniline
Galactooligosaccharides
Covalent immobilization
beta-galactosidase
Issue date2009
PublisherElsevier Ltd.
JournalReactive and Functional Polymers
CitationNERI, David F. M. [et al.] - Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles. “Reactive and Functional Polymers” [Em linha]. 69:4 (Apr. 2009) 246-251. [Consult. 25 Mar. 2009]. Disponível em WWW:<URL: http://www.sciencedirect.com/science?_ob= ArticleURL&_udi=B6TY0-4VDS8FN-1&_user= 2459786&_rdoc=1&_fmt=&_orig=search&_sort= d&view=c&_acct=C000057396&_version=1&_urlVersion=0&_userid=2459786&md5= c69b9a88ad4302f0fc6a009cb9b5e402>. ISSN 1381-5148.
Abstract(s)Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their time course production was described as a “bell-shaped” curve as a result of the balance between transgalactosylation and hydrolysis. No significative difference was observed for the free and immobilized enzymes. The reaction rates for lactose hydrolysis and GOS formation increased with increasing temperature from 30 °C to 60 °C, but GOS production at all lactose conversion levels was almost unchanged with changing temperature. The mPOS–PANI matrix was also characterized by scanning electronic microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), thermomagnetization, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA).
TypeArticle
URIhttp://hdl.handle.net/1822/8820
DOI10.1016/j.reactfunctpolym.2009.01.002
ISSN1381-5148
Publisher versionhttp://www.sciencedirect.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
Neri_Reactive_FunctionalPolymers[1].pdf648,26 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID