Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/8351

TitleUnravelling the role of Candida albicans secreted alcohols in the cross : talk with Candida parapsilosis
Author(s)Martins, Margarida Isabel Barros Coelho
Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
Issue dateOct-2008
PublisherTechnischen Universität München
CitationINTERNATIONAL BIOFILM CONFERENCE, 3, Munich, Germany, 2008 - “Proceedings of Biofilms III Conference”. [Munchen : Technischen Universität München, 2008]. p. 147-148.
Abstract(s)The success of Candida species as pathogens is attributed to survival strategies such as the ability to form biofilms. In fact, Candida albicans and Candida parapsilosis are frequently isolated solely or within mixed cultures from implanted devices and natural environments. Nevertheless, interactions between organisms, namely through signalling molecules, are not well understood. One of the challenges in cell-cell signalling science is to distinguish the types of communication between cells depending on the consequences to the sender and the responder cell. This study aimed to contribute to the elucidation of the role of recently characterized C. albicans secreted alcohols in C. parapsilosis biofilms development. C. parapsilosis ATCC 22019 biofilms were developed on the surface of microtriter plates. All experiments were performed in RPMI medium with cells grown at 37 °C, 130 rpm (initial cell density of 1 × 10 6 cells/ml). Commercial formulations (pro-analysis) of C. albicans secreted alcohols were added after 3 h of adhesion or to 48 h grown biofilms. Isoamylalcohol, 2-phenylethanol, 1-dodecanol, nerolidol and farnesol were independently assayed at physiological levels, and endpoints were determined at the end of 24 h. Biofilms were analysed in terms of cellular activity, by the reduction of a tetrazolium salt (XTT), and total biomass by crystal violet staining. Results indicated that, at initial biofilm stages, C. parapsilosis metabolic activity was significantly decreased by 2-phenylethanol, nerolidol and 1-dodecanol, while biofilm total biomass was not affected. At later stages of biofilm development, nerolidol induced significant reductions in biofilm cellular activity, while 1-dodecanol increased total biomass. Overall, these results indicate that a group of C. albicans secreted alcohols reduce C. parapsilosis cellular specific activity (defined as biofilm cell activity/ biofilm biomass). This suggests that these molecules may coerce C. parapsilosis into a non beneficial situation, which might give competitive advantages to C. albicans.
TypeAbstract
URIhttp://hdl.handle.net/1822/8351
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

Files in This Item:
File Description SizeFormat 
147-148[1].pdf438,78 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID