Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/74699

TitleChemically modified lipase from Thermomyces lanuginosus with enhanced esterification and transesterification activities
Author(s)Noro, Jennifer Martins
Cavaco-Paulo, Artur
Silva, C.
KeywordsLipase from Thermomyces lanuginosus
Dodecyl chains
Activity
Transesterification
Esterification
Issue date8-Nov-2021
PublisherWiley-Blackwell
JournalChemcatchem
CitationJennifer Noro; Cavaco-Paulo, Artur; Silva, Carla, Chemically modified lipase from Thermomyces lanuginosus with enhanced esterification and transesterification activities. ChemCatChem, 13(21), 4524-4531, 2021
Abstract(s)Lipase from Thermomyces lanuginosus is one of the most explored enzymes for the esterification of several added-value industrial compounds, such as biodiesel, fragrances, and flavors. Its selectivity in these reactions is mostly related with its activity towards small alcohols. In this work, the impact of the chemical modification, with 4 dodecyl chains at its surface, was evaluated regarding its transesterification and esterification activities, comparing with the native form. Linear size-differentiated alcohols (from 1 to 20 carbons in the aliphatic chain) were used to explore for the first time the effect of the chain length in both transesterification and esterification reactions, using p-nitrophenyl palmitate and oleic acid as model compounds, respectively. The chemically modified lipase showed an outstanding improvement of its catalytic performance than the native enzyme, being this increase directly proportional to the size of the alcohols chain used as substrates. The enormous potential and remarkable versatility of this novel super catalyst was here demonstrated, where diverse types of esters, differing in their potential applications (biodiesel, cosmetics, fine chemistry), were efficiently synthesized. The produced esters were fully characterized by 1H NMR, GC-MS, and FTIR.
TypeArticle
URIhttp://hdl.handle.net/1822/74699
DOI10.1002/cctc.202101050
ISSN1867-3880
e-ISSN1867-3899
Publisher versionhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1867-3899
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_54940_1.pdf
  Restricted access
1,12 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID