Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/73866

TitleAngiogenic potential of airbrushed fucoidan/polycaprolactone nanofibrous meshes
Author(s)Reys, Lara Priscila Lopes
Silva, Simone S.
Oliveira, C.
Neves, N. M.
Martins, Albino
Reis, R. L.
Silva, Tiago H.
KeywordsAirbrushing
Angiogenesis
Endothelial cells
Fucoidan
Nanofibrous meshes
Poly(E-caprolactone
Tissue engineering
Vascularization
Poly(epsilon-caprolactone)
Poly(ε-caprolactone)
Issue dateJul-2021
PublisherElsevier
JournalInternational Journal Of Biological Macromolecules
CitationReys L. L., Silva S. S., Oliveira C., Neves N. M., Martins A., Reis R. L., Silva T. H. Angiogenic potential of airbrushed fucoidan/polycaprolactone nanofibrous meshes, International Journal Of Biological Macromolecules, Vol. 183, pp. 695-706, doi:10.1016/j.ijbiomac.2021.04.166, 2021
Abstract(s)Implantation of biomaterials and hybrid constructs in tissue engineering approaches presents major limitations such as inflammatory reaction and the lack of vasculature integration. Therefore, new strategies are needed to enhance implants function, immune protection, and revascularization. In this work, we developed fibrous meshes composed by fucoidan (Fu), a sulfated polysaccharide extracted from brown algae, and polycaprolactone (PCL), a synthetic biodegradable polymer, using the airbrush technique. The chemical characterization by FTIR, EDS and XPS confirmed the presence of the two polymers in the structure of airbrushed nanofibrous meshes (ANFM). Moreover, these nanofibrous exhibited good wettability and mechanical properties envisaging their application as templates for biomaterials and cell culture. The developed ANFM were directly cultured with human pulmonary microvascular endothelial (HPMEC-ST1.6R) cells for up to 7 days. Biological results demonstrated that ANFM comprising Fu promoted cellular attachment, spreading, and proliferation of human endothelial cells. The angiogenic potential of ANFM was further evaluated by onplantation of PCL and PCL/Fu ANFM in chick chorioallantoic membrane (CAM). In ovo and ex ovo results showed that the incorporation of Fu increased the pro-angiogenic potential of ANFM. Altogether, the results suggest that airbrush biocomposite meshes could be used as a biomaterial substrate to promote vascularization.  
TypeArticle
URIhttp://hdl.handle.net/1822/73866
DOI10.1016/j.ijbiomac.2021.04.166
ISSN0141-8130
Publisher versionhttps://www.sciencedirect.com/science/article/pii/S0141813021009387
Peer-Reviewedyes
AccessEmbargoed access (2 Years)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
20526-published-angiogenic-potential-airbrushed-fucoidan.pdf
  Until 2023-08-01
2,56 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID