Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/73543

TitleEngineering 3D printed bioactive composite scaffolds based on the combination of aliphatic polyester and calcium phosphates for bone tissue regeneration
Author(s)Backes, Eduardo H.
Fernandes, Emanuel Mouta
Diogo, Gabriela S.
Marques, Catarina F.
Silva, Tiago H.
Costa, Lidiane C.
Passador, Fabio R.
Reis, R. L.
Pessan, Luiz A.
KeywordsAdditive manufacturing
Bioactive composites
Biodegradable polymer
Fused deposition modeling
Hydroxyapatite
β-TCP
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Issue dateMar-2021
PublisherElsevier
JournalMaterials Science & Engineering: C
CitationBackes, E. H., Fernandes, E. M., Diogo, G. S., Marques, C. F., Silva, T. H., Costa, L. C., ... & Pessan, L. A. (2021). Engineering 3D printed bioactive composite scaffolds based on the combination of aliphatic polyester and calcium phosphates for bone tissue regeneration. Materials Science and Engineering: C, 122, 111928
Abstract(s)In this study, polylactic acid (PLA) filled with hydroxyapatite (HA) or beta-tricalcium phosphate (TCP) in 5 wt% and 10 wt% of concentration were produced employing twin-screw extrusion followed by fused filament fabrication in two different architectures, varying the orientation of fibers of adjacent layers. The extruded 3D filaments presented suitable rheological and thermal properties to manufacture of 3D scaffolds envisaging bone tissue engineering. The produced scaffolds exhibited a high level of printing accuracy related to the 3D model; confirmed by micro-CT and electron microscopy analysis. The developed architectures presented mechanical properties compatible with human bone replacement. The addition of HA and TCP made the filaments bioactive, and the deposition of new calcium phosphates was observed upon 7 days of incubation in simulated body fluid, exemplifying a microenvironment suitable for cell attachment and proliferation. After 7 days of cell culture, the constructs with a higher percentage of HA and TCP demonstrated a significantly superior amount of DNA when compared to neat PLA, indicating that higher concentrations of HA and TCP could guide a good cellular response and increasing cell cytocompatibility. Differentiation tests were performed, and the biocomposites of PLA/HA and PLA/TCP exhibited earlier markers of cell differentiation as confirmed by alkaline phosphatase and alizarin red assays. The 3D printed composite scaffolds, manufactured with bioactive materials and adequate porous size, supported cell attachment, proliferation, and differentiation ,which together with their scalability, promise a high potential for bone tissue engineering applications.
TypeArticle
URIhttp://hdl.handle.net/1822/73543
DOI10.1016/j.msec.2021.111928
ISSN0928-4931
Publisher versionhttps://www.sciencedirect.com/science/article/pii/S0928493121000667
Peer-Reviewedyes
AccessEmbargoed access (1 Year)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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