Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/73531

TitleTumor-Associated protrusion fluctuations as a signature of cancer invasiveness
Author(s)Caballero, David
Brancato, V.
Lima, Ana Cláudia Fernandes
Abreu, C. M.
Neves, N. M.
Correlo, V. M.
Oliveira, J. M.
Reis, R. L.
Kundu, S. C.
KeywordsBiophysics
Cancer
Invasion
Prediction
Protrusions
biophysical signatures
fluctuations
micro-spheroids
tumors invasion
Issue dateJul-2021
PublisherWiley
JournalAdvanced Biology
CitationCaballero D., Brancato V., Lima Ana C., Abreu C. M., Neves N. M., Correlo V. M., Oliveira J. M., Reis R. L., Kundu S. C. Tumor-Associated Protrusion Fluctuations as a Signature of Cancer Invasiveness, Advanced Biology, pp. 2101019, doi:10.1002/adbi.202101019, 2021
Abstract(s)The generation of invasive fluctuating protrusions is a distinctive feature of tumor dissemination. During the invasion, individual cancer cells modulate the morphodynamics of protrusions to optimize their migration efficiency. However, it remains unclear how protrusion fluctuations govern the invasion of more complex multi-cellular structures, such as tumors, and their correlation with the tumor metastatic potential. Herein, a reductionist approach based on 3D tumor cell micro-spheroids with different invasion capabilities is used as a model to decipher the role of tumor-associated fluctuating protrusions in cancer progression. To quantify fluctuations, a set of key biophysical parameters that precisely correlate with the invasive potential of tumors is defined. It is shown that different pharmacological drugs and cytokines are capable of modulating protrusion activity, significantly altering protrusion fluctuations, and tumor invasiveness. This correlation is used to define a novel quantitative invasion index encoding the key biophysical parameters of fluctuations and the relative levels of cell-cell/matrix interactions, which is capable of assessing the tumor's metastatic capability solely based on its magnitude. Overall, this study provides new insights into how protrusion fluctuations regulate tumor cell invasion, suggesting that they may be employed as a novel early indicator, or biophysical signature, of the metastatic potential of tumors.
TypeArticle
Descriptionuncorrected proof version
URIhttp://hdl.handle.net/1822/73531
DOI10.1002/adbi.202101019
ISSN2701-0198
Publisher versionhttps://onlinelibrary.wiley.com/doi/10.1002/adbi.202101019
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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