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TitleHorseradish peroxidase-crosslinked calcium-containing silk fibroin hydrogels as artificial matrices for bone cancer research
Author(s)Pierantoni, Lara
Ribeiro, Viviana Pinto
Costa, Lígia Francisca Rodrigues
Pina, Sandra Cristina Almeida
da Silva Morais, Alain
Silva-Correia, Joana
Kundu, Subhas C
Motta, Antonella
Reis, R. L.
Oliveira, J. M.
Silk Fibroin
Issue dateFeb-2021
JournalMacromolecular Bioscience
CitationPierantoni L., Ribeiro V. P., Costa L., Pina S., da Silva Morais A., Silva-Correia J., Kundu S. C., Motta A., Reis R. L., Oliveira J. M. Horseradish Peroxidase-Crosslinked Calcium-containing Silk Fibroin Hydrogels as Artificial Matrices for Bone Cancer Research, Macromolecular Bioscience, doi:10.1002/mabi.202000425, 2021
Abstract(s)Hydrogels, being capable of mimicking the extracellular matrix composition of tissues, are greatly used as artificial matrices in tissue engineering applications. In this study, we report the generation of horseradish peroxidase (HRP)-crosslinked silk fibroin (SF) hydrogels, using calcium peroxide as oxidizer. The proposed fast forming calcium-containing SF hydrogels spontaneously undergo SF conformational changes from random coil to β-sheet during time, exhibiting ionic and pH stimuli responsiveness. In vitro response shows calcium-containing SF hydrogelsâ encapsulation properties and their ability to promote SaOs-2 tumor cells death after 10 days of culturing, upon complete β-sheet conformation transition. Calcium-containing SF hydrogelsâ angiogenic potential investigated in an in ovo chick chorioallantoic membrane (CAM) assay, show a high number of converging blood vessels as compared to the negative control, although no endothelial cells infiltration is observed. The in vivo response evaluated in subcutaneous implantation in CD1 and nude NCD1 mice shows that calcium-containing SF hydrogels are stable up to 6 weeks after implantation. However, an increased number of dead cells are also present in the surrounding tissue. The results suggest the potential of calcium-containing SF hydrogels to be used as novel in situ therapeutics for bone cancer treatment applications, particularly to osteosarcoma.
Description"Online ahead of print"
Publisher version10.1002/mabi.202000425
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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