Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/69201

TitleCytotoxic plant extracts towards insect cells: bioactivity and nanoencapsulation studies for application as biopesticides
Author(s)Lopes, Ana I. F.
Monteiro, Mariana
Araújo, Ana Rita L.
Rodrigues, Ana Rita Oliveira
Castanheira, Elisabete M. S.
Pereira, David M.
Olim, Pedro
Fortes, A. Gil
Gonçalves, M. Sameiro T.
Keywordsplant extracts
nanoencapsulation
biopesticides
bioactivity
bioinsecticides
Ruta graveolens
chlorpyrifos
Issue dateDec-2020
PublisherMDPI
JournalMolecules
CitationLopes, A.I.F.; Monteiro, M.; Araújo, A.R.L.; Rodrigues, A.R.O.; Castanheira, E.M.S.; Pereira, D.M.; Olim, P.; Fortes, A.G.; Gonçalves, M.S.T. Cytotoxic Plant Extracts towards Insect Cells: Bioactivity and Nanoencapsulation Studies for Application as Biopesticides. Molecules 2020, 25, 5855.
Abstract(s)The potential of plant extracts as bioinsecticides has been described as a promising field of agricultural development. In this work, the extracts of Punica granatum (pomegranate), Phytolacca americana (American pokeweed), Glandora prostrata (shrubby gromwell), Ulex europaeus (gorce), Tagetes patula (French marigold), Camellia japonica red (camellia), Ruta graveolens (rue or herb-of-grace) were obtained, purified, and their activity against Spodoptera frugiperda (Sf9) insect cells was investigated. From the pool of over twenty extracts obtained, comprising different polarities and vegetable materials, less polar samples were shown to be more toxic towards the insect cell line Sf9. Among these, a dichloromethane extract of R. graveolens was capable of causing a loss of viability of over 50%, exceeding the effect of the commercial insecticide chlorpyrifos. This extract elicited chromatin condensation and the fragmentation in treated cells. Nanoencapsulation assays of the cytotoxic plant extracts in soybean liposomes and chitosan nanostructures were carried out. The nanosystems exhibited sizes lower or around 200 nm, low polydispersity, and generally high encapsulation efficiencies. Release assays showed that chitosan nanoemulsions provide a fast and total extract release, while liposome-based systems are suitable for a more delayed release. These results represent a proof-of-concept for the future development of bioinsecticide nanoformulations based on the cytotoxic plant extracts.
TypeArticle
URIhttp://hdl.handle.net/1822/69201
DOI10.3390/molecules25245855
e-ISSN1420-3049
Publisher versionhttps://www.mdpi.com/1420-3049/25/24/5855
Peer-Reviewedyes
AccessOpen access
Appears in Collections:PHYSICS OF QUANTUM MATERIALS AND BIONANOSTRUCTURES (2018 - ...)
CDQuim - Artigos (Papers)

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