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TitleEpitope‐imprinted nanoparticles as transforming growth factor‐β3 sequestering ligands to modulate stem cell fate
Author(s)Teixeira S. P. B.
Domingues, R. M. A.
Babo, Pedro Miguel Sousa
Berdecka, D.
Miranda, Margarida Silva
Gomes, Manuela E.
Peppas, N. A.
Reis, R. L.
Molecular imprinting
Molecular recognition
protein corona
Transforming growth factor-β3 (TGF-β3)
transforming growth factor-β3
transforming growth factor-beta 3
Issue dateOct-2020
JournalAdvanced Functional Materials
CitationTeixeira S. P. B., Domingues R. M. A., Babo P. S., Berdecka D., Miranda M. S., Gomes M. E., Peppas N. A., Reis R. L. Epitope‐Imprinted Nanoparticles as Transforming Growth Factor‐β3 Sequestering Ligands to Modulate Stem Cell Fate, Advanced Functional Materials, pp. 2003934, doi:10.1002/adfm.202003934, 2020
Abstract(s)Growth factors (GFs) are biomolecules with potent biological effects but inherent limitations hinder their potential as therapeutic agents and cell culture supplements in tissue engineering and regenerative medicine (TERM). Biomaterials that sequester endogenous GFs by affinity binding might circumvent such limitations and thus are being increasingly investigated. Here, molecularly imprinted nanoparticles (MINPs) are proposed as specific abiotic ligands for GFs. As a proof of concept, a conformational epitope of transforming growth factorâ β3 (TGFâ β3) is designed and surface imprinted onto polyacrylamideâ based nanoparticles by inverse microemulsion polymerization. It is found that, depending on the polymerization mixture composition, MINPs can recognize and preferentially bind TGFâ β3, either in noncompetitive assays or from a complex human fluid (platelet lysate). Substrates functionalized with MINPs are then used for 2D culture of adiposeâ derived stem cells. Remarkably, gene and protein expression profiles show a marked upregulation of SOXâ 9, suggesting activation of TGFâ β3 signaling pathways without requiring supplementation with exogenous GF. Likewise, culturing these cells in pellets incorporating MINPs previously incubated with platelet lysate results in higher collagen IIâ rich matrix deposition, compared to pellets incorporating nonâ imprinted nanoparticles. In summary, results suggest MINPs can be used as costâ effective, stable, and scalable alternative abiotic GF ligands to guide cell fate in TERM applications.
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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