Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/68263

TitleIncreased intraspecies diversity in Escherichia coli biofilms promotes cellular growth at the expense of matrix production
Author(s)Azevedo, Andreia S.
Gerola, Gislaine P.
Baptista, João
Almeida, Carina
Peres, Joana
Mergulhão, Filipe J.
Azevedo, Nuno F.
Keywordsbiofilms
Escherichia coli
intraspecies community
EPS matrix
peptide nucleic acid-fluorescence in situ hybridization
urinary tract infections
catheter-associated urinary tract infections
confocal laser scanning microscopy
Issue date2020
PublisherMDPI AG
JournalAntibiotics
CitationAzevedo, Andreia S.; Gerola, Gislaine P.; Baptista, João; Almeida, Carina; Peres, Joana; Mergulhão, Filipe J.; Azevedo, Nuno F., Increased intraspecies diversity in Escherichia coli biofilms promotes cellular growth at the expense of matrix production. Antibiotics, 9(11), 818, 2020
Abstract(s)Intraspecies diversity in biofilm communities is associated with enhanced survival and growth of the individual biofilm populations. Studies on the subject are scarce, namely, when more than three strains are present. Hence, in this study, the influence of intraspecies diversity in biofilm populations composed of up to six different Escherichia coli strains isolated from urine was evaluated in conditions mimicking the ones observed in urinary tract infections and catheter-associated urinary tract infections. In general, with the increasing number of strains in a biofilm, an increase in cell cultivability and a decrease in matrix production were observed. For instance, single-strain biofilms produced an average of 73.1 µg·cm−2 of extracellular polymeric substances (EPS), while six strains biofilms produced 19.9 µg·cm−2. Hence, it appears that increased genotypic diversity in a biofilm leads E. coli to direct energy towards the production of its offspring, in detriment of the production of public goods (i.e., matrix components). Apart from ecological implications, these results can be explored as another strategy to reduce the biofilm burden, as a decrease in EPS matrix production may render these intraspecies biofilms more sensitive to antimicrobial agents.
TypeArticle
URIhttp://hdl.handle.net/1822/68263
DOI10.3390/antibiotics9110818
e-ISSN2079-6382
Publisher versionhttps://www.mdpi.com/journal/antibiotics
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_53996_1.pdf1,47 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID