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https://hdl.handle.net/1822/67443
Título: | Gene expression during activation of Paracoccidioides brasiliensis conidia |
Autor(es): | Hernandez, O. Garcia, A. M. Almeida, Agostinho João Ramalho Tamayo, D. Gonzalez, A. Restrepo, A. McEwen, J. G. |
Palavras-chave: | Fungal Proteins Mycelium Paracoccidioides Spores, Fungal Gene Expression Regulation, Fungal P. brasiliensis Transition and germination Gene expression |
Data: | Nov-2011 |
Editora: | Wiley |
Revista: | Yeast |
Citação: | Hernandez, O., Garcia, A. M., Almeida, A. J., Tamayo, D., Gonzalez, A., Restrepo, A., & McEwen, J. G. (2011). Gene expression during activation of Paracoccidioides brasiliensis conidia. Yeast, 28(11), 771-781 |
Resumo(s): | This study focuses on gene expression during crucial biological phenomena of the dimorphic fungal human pathogen Paracoccidioides brasiliensis, the conidia-to-yeast (C-Y) transition and the conidia-to-mycelia (C-M) germination. We studied 10 genes involved in different cellular functions: oxidative stress response (alternative oxidase (AOX), superoxide dismutase (SOD), flavodoxin, conserved hypothetical protein (Y20)); cell metabolism (glyceraldehyde-3-phosphate dehydrogenase (GADPH), cholestenol Delta-isomerase (ChDI), glycine dehydrogenase (GDh)) and heat shock response (Heat shock protein 90 (HSP90)), and cell synthesis and wall structure (glucan synthase-1 (GS-1), α-1,3-glucan synthase (αGS), and mannosyltransferase (MT)). Gene expression was measured during the first 72 h and 96 h of C-Y and C-M, respectively, previously shown to be a fundamental time frame for the consolidation of these cellular processes. The gene expression of AOX, GAPDH, HSP90, MT, αGS, and GDh was significantly increased during the C-Y transition, while SOD, ChDI, GAPDH, MT, GDh, and GS-1 were increased during C-M germination. Additionally, some were highly expressed in each process: AOX, HSP90, and αGS during C-Y; SOD, ChDI, and GS-1 during C-M. Altogether, these data add new information regarding gene expression during the C-Y and C-M processes. Future research will be targeted to further characterize the true relevance of the studied genes during the morphological transition, either during adaptation to the environment or to the infected host. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67443 |
DOI: | 10.1002/yea.1902 |
ISSN: | 0749-503X |
e-ISSN: | 1097-0061 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1002/yea.1902 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Hernandez-2011-Gene-expression-during-activation-o.pdf Acesso restrito! | 308,71 kB | Adobe PDF | Ver/Abrir |