Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/6738

TitleApplication of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
Author(s)Ribeiro, Orquídea
Gombert, Andreas Karoly
Teixeira, J. A.
Domingues, Lucília
KeywordsCre-loxP system
Kluyveromyces marxianus
Gene disruption
β-Galactosidase
Gene targeting efficiency
beta-galactosidase
Issue dateAug-2007
PublisherElsevier B.V.
JournalJournal of Biotechnology
CitationRIBEIRO, Orquídea [et al.] - Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus. "Journal of Biotechnology" [Em linha]. 131:1 (Ago. 2007) 20-26. [Consult. 19 Jul. 2007. Disponível em WWW:<URL: http://www.sciencedirect.com/science/journal/01681656. ISSN 0168-1656.
Abstract(s)The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity.
TypeArticle
URIhttp://hdl.handle.net/1822/6738
DOI10.1016/j.jbiotec.2007.05.027
ISSN0168-1656
Publisher versionhttp://www.sciencedirect.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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