Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/66344
Título: | Mineralization of layer-by-layer ultrathin films containing microfluidic-produced hydroxyapatite nanorods |
Autor(es): | Rial, R. Costa, Rui Filipe Ramos Reis, R. L. Liu, Z. Pashkuleva, I. Ruso, J. M. |
Palavras-chave: | electrostatic interactions hydroxyapatite layer-by-layer Microfluidics osteoconductivity |
Data: | Set-2019 |
Editora: | ACS |
Revista: | Crystal Growth & Design |
Citação: | Rial R., Costa R. R., Reis R. L., Liu Z., Pashkuleva I., Ruso J. M. Mineralization of layer-by-layer ultrathin films containing microfluidic-produced hydroxyapatite nanorods, Crystal Growth & Design, Vol. 19, Issue 11, pp. 6351 - 6359, doi:10.1021/acs.cgd.9b00831, 2019 |
Resumo(s): | We describe the assembly of layer-by-layer (LbL) ultrathin films containing bioactive hydroxyapatite (HAp) rod-shaped nanoparticles with mineralizing capacity. Monodisperse 96 nm long and 9 nm wide HAp nanorods with a surface charge of â 14 mV were produced with a microfluidic system. The negatively charged HAp nanorods were assembled with the polycation poly-L-lysine (PLL) in LbL fashion. The successful deposition of alternating layers was confirmed by quartz-crystal microbalance with dissipation monitoring. The Voigt-based viscoelastic model demonstrated steady film growth where three PLL/HAp bilayers reached a thickness of 70 nm. The bioactivity of [PLL/HAp]3 was evaluated in vitro by following the formation of a mineralized hydroxyapatite layer in simulated body fluid (SBF). X-ray diffraction, energy-dispersive X-ray spectroscopy and scanning electron microscopy (SEM) demonstrated formation of a crystalline hydroxyapatite layer and complete surface coverage within 7 days. SaOs-2 osteoblasts-like cells attached to the mineralized surfaces and developed longer filopodia extensions when compared to non-mineralized samples. Our results showed that [PLL/HAp]3 films are feasible osteoconductive coatings applicable to orthopedic implants and fixation devices. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/66344 |
DOI: | 10.1021/acs.cgd.9b00831 |
ISSN: | 1528-7483 |
Versão da editora: | https://pubs.acs.org/doi/10.1021/acs.cgd.9b00831 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19925-acs.cgd_.9b00831.pdf | 41,19 MB | Adobe PDF | Ver/Abrir |