Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/66342

TitleSardine roe as a source of lipids to produce liposomes
Author(s)Guedes, M.
Costa-Pinto, A. R.
Gonçalves, V. M. F.
Moreira-Silva, J.
Tiritan, M. E.
Reis, R. L.
Ferreira, H.
Neves, N. M.
KeywordsBligh and Dyer
Cytocompatibility
fatty acids
Liposomes
methyl-tert-butyl ether
sardine roe
methyl-tent-butyl ether
Issue dateJan-2020
PublisherAmerican Chemical Society
JournalACS Biomaterials Science & Engineering
CitationGuedes M., Costa-Pinto A. R., Gonçalves V. M. F., Moreira-Silva J., Tiritan M. E., Reis R. L., Ferreira H., Neves N. M. Sardine Roe as a Source of Lipids To Produce Liposomes, ACS Biomaterials Science & Engineering, Vol. 6, Issue 2, pp. 1017–1029, doi:10.1021/acsbiomaterials.9b01462, 2020
Abstract(s)Sea-derived materials have promising applications in the medical, pharmaceutical, and biotechnological fields. Fish roe, for example, is a highly nutritional product, presenting diverse beneficial effects on human health. Therefore, this work explored extracts of sardine (Sardina pilchardus) roe, due to the well-known health benefits of this fish, to produce novel and promising delivery systems. After morphological, histological, and histochemical characterizations of sardine roe, their lipids were extracted using two different approaches, namely, Bligh and Dyer (BD) and methyl-tert-butyl ether (MTBE) methods. Gas chromatography/mass spectrometry analyses demonstrated that lipid extracts contain several fatty acids, such as Ï 3 polyunsaturated fatty acids. The lipids, especially phospholipids, were used to produce multilamellar liposomes (MLVs). These delivery systems presented size heterogeneity, a negative surface charge, and the ability to control the release of the encapsulated anti-inflammatory drug, namely, celecoxib. Biological assays indicated that MLVs produced with MTBE lipidic extracts presented a better cytocompatibility than those obtained by the BD method. This can be further improved if the lipid extracts are processed by chemical extraction. Therefore, sardine roe-derived lipids can produce drug-delivery systems with the potential to be applied in the biomedical field.
TypeArticle
URIhttp://hdl.handle.net/1822/66342
DOI10.1021/acsbiomaterials.9b01462
ISSN2373-9878
Publisher versionhttps://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.9b01462
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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