Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/66189

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dc.contributor.authorDencheva, Nadyapor
dc.contributor.authorBraz, Joanapor
dc.contributor.authorScheibel, Dieterpor
dc.contributor.authorMalfois, Marcpor
dc.contributor.authorDenchev, Z.por
dc.contributor.authorGitsov, Ivanpor
dc.date.accessioned2020-07-30T14:18:17Z-
dc.date.available2020-07-30T14:18:17Z-
dc.date.issued2020-
dc.identifier.citationDencheva, N.; Braz, J.; Scheibel, D.; Malfois, M.; Denchev, Z.; Gitsov, I. Polymer-Assisted Biocatalysis: Polyamide 4 Microparticles as Promising Carriers of Enzymatic Function. Catalysts 2020, 10, 767.por
dc.identifier.urihttp://hdl.handle.net/1822/66189-
dc.description.abstractThis study reports a new strategy for enzyme immobilization based on passive immobilization in neat and magnetically responsive polyamide 4 (PA4) highly porous particles. The microsized particulate supports were synthesized by low-temperature activated anionic ring-opening polymerization. The enzyme of choice was laccase from <i>Trametes versicolor</i> and was immobilized by either adsorption on prefabricated PA4 microparticles (PA4@iL) or by physical in situ entrapment during the PA4 synthesis (PA4@eL). The surface topography of all PA4 particulate supports and laccase conjugates, as well as their chemical and physical structure, were studied by microscopic, spectral, thermal, and synchrotron WAXS/SAXS methods. The laccase content and activity in each conjugate were determined by complementary spectral and enzyme activity measurements. PA4@eL samples displayed >93% enzyme retention after five incubation cycles in an aqueous medium, and the PA4@iL series retained ca. 60% of the laccase. The newly synthesized PA4-laccase complexes were successfully used in dyestuff decolorization aiming at potential applications in effluent remediation. All of them displayed excellent decolorization of positively charged dyestuffs reaching ~100% in 15 min. With negative dyes after 24 h the decolorization reached 55% for PA4@iL and 85% for PA4@eL. A second consecutive decolorization test revealed only a 5–10% decrease in effectiveness indicating the reusability potential of the laccase-PA4 conjugates.por
dc.description.sponsorshipThe authors gratefully acknowledge the financial support of the project TSSiPRONORTE-01-0145-FEDER-000015, supported by the regional operation program NORTE2020, under the Portugal2020 Partnership Agreement, through the European Regional Development Fund, as well as the support byNational Funds throughFundação para a Ciência e Tecnologia(FCT), project UID/CTM/50025/2019. D.S. and I.G.wish to thank the Research Foundation of the State of New York for partial funding through Materials Network ofExcellence. N.D. is also grateful for the personal program-contract CTTI-51/18-IPC.por
dc.language.isoengpor
dc.publisherMultidisciplinary Digital Publishing Institutepor
dc.relationUID/CTM/50025/2019por
dc.rightsopenAccesspor
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/por
dc.subjectlaccasepor
dc.subjectpolyamide 4por
dc.subjectenzyme immobilizationpor
dc.subjectmagnetic enzyme supportspor
dc.subjectenzyme decolorizationpor
dc.titlePolymer-assisted biocatalysis: polyamide 4 microparticles as promising carriers of enzymatic functionpor
dc.typearticlepor
dc.peerreviewedyespor
dc.relation.publisherversionhttps://www.mdpi.com/2073-4344/10/7/767por
dc.date.updated2020-07-24T13:37:20Z-
dc.identifier.essn2073-4344-
dc.identifier.doi10.3390/catal10070767por
sdum.journalCatalystspor
oaire.versionVoRpor
Appears in Collections:IPC - Artigos em revistas científicas internacionais com arbitragem

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