Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/66189

TitlePolymer-assisted biocatalysis: polyamide 4 microparticles as promising carriers of enzymatic function
Author(s)Dencheva, Nadya
Braz, Joana
Scheibel, Dieter
Malfois, Marc
Denchev, Z.
Gitsov, Ivan
Keywordslaccase
polyamide 4
enzyme immobilization
magnetic enzyme supports
enzyme decolorization
Issue date2020
PublisherMultidisciplinary Digital Publishing Institute
JournalCatalysts
CitationDencheva, N.; Braz, J.; Scheibel, D.; Malfois, M.; Denchev, Z.; Gitsov, I. Polymer-Assisted Biocatalysis: Polyamide 4 Microparticles as Promising Carriers of Enzymatic Function. Catalysts 2020, 10, 767.
Abstract(s)This study reports a new strategy for enzyme immobilization based on passive immobilization in neat and magnetically responsive polyamide 4 (PA4) highly porous particles. The microsized particulate supports were synthesized by low-temperature activated anionic ring-opening polymerization. The enzyme of choice was laccase from <i>Trametes versicolor</i> and was immobilized by either adsorption on prefabricated PA4 microparticles (PA4@iL) or by physical in situ entrapment during the PA4 synthesis (PA4@eL). The surface topography of all PA4 particulate supports and laccase conjugates, as well as their chemical and physical structure, were studied by microscopic, spectral, thermal, and synchrotron WAXS/SAXS methods. The laccase content and activity in each conjugate were determined by complementary spectral and enzyme activity measurements. PA4@eL samples displayed >93% enzyme retention after five incubation cycles in an aqueous medium, and the PA4@iL series retained ca. 60% of the laccase. The newly synthesized PA4-laccase complexes were successfully used in dyestuff decolorization aiming at potential applications in effluent remediation. All of them displayed excellent decolorization of positively charged dyestuffs reaching ~100% in 15 min. With negative dyes after 24 h the decolorization reached 55% for PA4@iL and 85% for PA4@eL. A second consecutive decolorization test revealed only a 5–10% decrease in effectiveness indicating the reusability potential of the laccase-PA4 conjugates.
TypeArticle
URIhttp://hdl.handle.net/1822/66189
DOI10.3390/catal10070767
e-ISSN2073-4344
Publisher versionhttps://www.mdpi.com/2073-4344/10/7/767
Peer-Reviewedyes
AccessOpen access
Appears in Collections:IPC - Artigos em revistas científicas internacionais com arbitragem

Files in This Item:
File Description SizeFormat 
catalysts-10-00767-v3.pdf6,5 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID