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https://hdl.handle.net/1822/65787
Título: | Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids |
Autor(es): | Kurnik, Isabelle S. Noronha, Mariana A. Câmara, Mayra C. C. Mazzola, Priscila G. Vicente, A. A. Pereira, Jorge F. B. Lopes, André M. |
Palavras-chave: | curcumin (CCM) separation Pluronic F68 polymeric micelles (PMs) aqueous two-phase micellar systems (ATPMS) ionic liquids (ILs) |
Data: | Nov-2020 |
Editora: | Elsevier |
Revista: | Separation and Purification Technology |
Citação: | Kurnik, Isabelle S.; Noronha, Mariana A.; Câmara, Mayra C. C.; Mazzola, Priscila G.; Vicente, António A.; Pereira, Jorge F. B.; Lopes, André M., Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids. Separation and Purification Technology, 250(117262), 2020 |
Resumo(s): | Novel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). CCM stability in the presence of ATPMS components was also evaluated. CCM is stable up to 24 h in copolymer (1.0 10.0 wt%) and ILs (0.1 3.0 M) aqueous solutions. Very mild phase separation conditions (close to room temperature) were achieved by adding cholinium ILs to the Pluronic F68 + McIlvaine buffer at pH 6.0 solution. The decrease of cloud-point temperature is dependent on the relative hydrophobicity of IL anion, [Hex] > [But] > [Prop] > [Ac] > Cl. ATPMS composed of more hydrophobic ILs ([Ch][Hex] > [Ch][But] > [Ch][Prop]) are most efficient in the partition of commercial CCM into polymeric micelles-rich phase. The best ATPMS (0.70 M [Ch][But] and 0.60 M [Ch][Hex]-based ATPMS) were then used to purify CCM from a crude extract of Curcuma longa L. Both systems were very selective to separate CCM from protein-based contaminants (selectivity values 25; purification yields 12-fold). Pluronic F68-based ATPMS are promising for selective separation of hydrophobic biomolecules by using cholinium-based ILs as adjuvants to adjust phase separation temperatures and biomolecules partition. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/65787 |
DOI: | 10.1016/j.seppur.2020.117262 |
ISSN: | 1383-5866 |
e-ISSN: | 1383-5866 |
Versão da editora: | https://www.journals.elsevier.com/separation-and-purification-technology |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53771_1.pdf | 6,42 MB | Adobe PDF | Ver/Abrir |