Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/65698

TitlePontiella desulfatans gen. nov., sp. nov., and Pontiella sulfatireligans sp. nov., two marine anaerobes of the Pontiellaceae fam. nov. producing sulfated glycosaminoglycan-like exopolymers
Author(s)van Vliet, Daan M.
Lin, Yuemei
Bale, Nicole J.
Koenen, Michel
Villanueva, Laura
Stams, Alfons Johannes Maria
Sánchez-Andrea, Irene
Keywordsnovel anaerobes
CAZymes
glycosaminoglycans
Kiritimatiella
Black Sea
Issue date18-Jun-2020
PublisherMDPI Publishing
JournalMicroorganisms
Citationvan Vliet, Daan M.; Lin, Yuemei; Bale, Nicole J.; Koenen, Michel; Villanueva, Laura; Stams, A. J. M.; Sánchez-Andrea, Irene, Pontiella desulfatans gen. nov., sp. nov., and Pontiella sulfatireligans sp. nov., two marine anaerobes of the Pontiellaceae fam. nov. producing sulfated glycosaminoglycan-like exopolymers. Microorganisms, 8(6), 920, 2020
Abstract(s)Recently, we isolated two marine strains, F1T and F21T, which together with Kiritimatiella glycovorans L21-Fru-ABT are the only pure cultures of the class Kiritimatiellae within the phylum Verrucomicrobiota. Here, we present an in-depth genome-guided characterization of both isolates with emphasis on their exopolysaccharide synthesis. The strains only grew fermentatively on simple carbohydrates and sulfated polysaccharides. Strains F1T, F21T and K. glycovorans reduced elemental sulfur, ferric citrate and anthraquinone-2,6-disulfonate during anaerobic growth on sugars. Both strains produced exopolysaccharides during stationary phase, probably with intracellularly stored glycogen as energy and carbon source. Exopolysaccharides included N-sulfated polysaccharides probably containing hexosamines and thus resembling glycosaminoglycans. This implies that the isolates can both degrade and produce sulfated polysaccharides. Both strains encoded an unprecedently high number of glycoside hydrolase genes (422 and 388, respectively), including prevalent alpha-L-fucosidase genes, which may be necessary for degrading complex sulfated polysaccharides such as fucoidan. Strain F21T encoded three putative glycosaminoglycan sulfotransferases and a putative sulfate glycosaminoglycan biosynthesis gene cluster. Based on phylogenetic and chemotaxonomic analyses, we propose the taxa Pontiella desulfatans F1T gen. nov., sp. nov. and Pontiella sulfatireligans F21T sp. nov. as representatives of the Pontiellaceae fam. nov. within the class Kiritimatiellae.
TypeArticle
URIhttp://hdl.handle.net/1822/65698
DOI10.3390/microorganisms8060920
e-ISSN2076-2607
Publisher versionhttps://www.mdpi.com/2076-2607/8/6/920
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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