Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/65510
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Monteiro-Reis, Sara | por |
dc.contributor.author | Lameirinhas, Ana | por |
dc.contributor.author | Miranda-Gonçalves, Vera | por |
dc.contributor.author | Felizardo, Diana | por |
dc.contributor.author | Dias, Paula C. | por |
dc.contributor.author | Oliveira, Jorge | por |
dc.contributor.author | Graça, Inês | por |
dc.contributor.author | Gonçalves, Celine Saraiva | por |
dc.contributor.author | Costa, Bruno Marques | por |
dc.contributor.author | Henrique, Rui | por |
dc.contributor.author | Jerónimo, Carmen | por |
dc.date.accessioned | 2020-05-28T17:57:23Z | - |
dc.date.available | 2020-05-28T17:57:23Z | - |
dc.date.issued | 2020-04-25 | - |
dc.identifier.citation | Monteiro-Reis, S.; Lameirinhas, A.; Miranda-Gonçalves, V.; Felizardo, D.; Dias, P.C.; Oliveira, J.; Graça, I.; Gonçalves, C.S.; Costa, B.M.; Henrique, R.; Jerónimo, C. Sirtuins’ Deregulation in Bladder Cancer: SIRT7 Is Implicated in Tumor Progression through Epithelial to Mesenchymal Transition Promotion. Cancers 2020, 12, 1066. | por |
dc.identifier.uri | https://hdl.handle.net/1822/65510 | - |
dc.description.abstract | Sirtuins are emerging players in cancer biology and other age-related disorders, and their putative role in bladder cancer (BlCa) remains elusive. Further understanding of disease biology may allow for generation of more effective pathway-based biomarkers and targeted therapies. Herein, we aimed to illuminate the role of sirtuins’ family in BlCa and evaluate their potential as disease biomarkers and therapeutic targets. SIRT1-7 transcripts and protein levels were evaluated in a series of primary BlCa and normal bladder mucosa tissues. SIRT7 knockdown was performed through lentiviral transduction in MGHU3, 5637 and J82 cells and its functional role was assessed. SIRT1, 2, 4 and 5 expression levels were significantly lower in BlCa, whereas SIRT6 and 7 were overexpressed, and these results were corroborated by TCGA cohort analysis. SIRT7 transcript levels were significantly decreased in muscle-invasive vs. papillary BlCa. In vitro studies showed that SIRT7 downregulation promoted cells migration and invasion. Accordingly, increased EMT markers expression and decreased E-Cadherin (CDH1) was observed in those BlCa cells. Moreover, increased EZH2 expression and H3K27<sup>me3</sup> deposition in E-Cadherin promoter was found in sh-SIRT7 cells. We demonstrated that sirtuins are globally deregulated in BlCa, and specifically SIRT7 downregulation is implicated in EMT, fostering BlCa invasiveness through EZH2-CDH1 axis. | por |
dc.description.sponsorship | This research was supported by the Research Center of the Portuguese Oncology Institute of Porto (CI-IPOP–FBGEBC-27 and PI 74-CI-IPOP-19-2016), by Fundação para a Ciência e Tecnologia (FCT) (PhD fellowships SFRH/BD/112673/2015 to S.M.-R and SFRH/BD/92786/2013 to C.S.G.; IF/00601/2012 to B.M.C.), and by Fundo Europeu de Desenvolvimento Regional (FEDER) (post-doctoral fellowships IPO/ESTIMANORTE01-0145-FEDER-000027 to V.M.-G. and COMPETE/FEDER/FCT_CI-IPOP-BPD/UID/DTP/00776/2013 to I.G.). | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute | por |
dc.relation | SFRH/BD/112673/2015 | por |
dc.relation | SFRH/BD/92786/2013 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147346/PT | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | bladder cancer | por |
dc.subject | SIRT7 | por |
dc.subject | EMT | por |
dc.title | Sirtuins’ deregulation in bladder cancer: SIRT7 is implicated in tumor progression through epithelial to mesenchymal transition promotion | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2072-6694/12/5/1066 | por |
oaire.citationIssue | 5 | por |
oaire.citationVolume | 12 | por |
dc.date.updated | 2020-05-28T14:08:06Z | - |
dc.identifier.eissn | 2072-6694 | - |
dc.identifier.doi | 10.3390/cancers12051066 | por |
dc.subject.fos | Ciências Médicas::Ciências da Saúde | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Cancers | por |
oaire.version | VoR | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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cancers-12-01066-v2.pdf | 3,13 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons