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TitleMathematical modelling using predictive biomarkers for the outcome of canine Leishmaniasis upon chemotherapy
Author(s)Gonçalves, Rafaela de Sousa
Alves de Pinho, Flaviane
Dinis-Oliveira, Ricardo Jorge
Azevedo, Rui
Gaifem, Joana
Farias Larangeira, Daniela
Ramos-Sanchez, Eduardo Milton
Goto, Hiro
Silvestre, Ricardo
Barrouin-Melo, Stella Maria
Keywordsmathematical model
hematological parameters
biochemical parameters
Issue date15-May-2020
PublisherMultidisciplinary Digital Publishing Institute
Citationde Sousa Gonçalves, R.; Alves de Pinho, F.; Dinis-Oliveira, R.J.; Azevedo, R.; Gaifem, J.; Farias Larangeira, D.; Ramos-Sanchez, E.M.; Goto, H.; Silvestre, R.; Barrouin-Melo, S.M. Mathematical Modelling Using Predictive Biomarkers for the Outcome of Canine Leishmaniasis upon Chemotherapy. Microorganisms 2020, 8, 745.
Abstract(s)Prediction parameters of possible outcomes of canine leishmaniasis (CanL) therapy might help with therapeutic decisions and animal health care. Here, we aimed to develop a diagnostic method with predictive value by analyzing two groups of dogs with CanL, those that exhibited a decrease in parasite load upon antiparasitic treatment (group: responders) and those that maintained high parasite load despite the treatment (group: non-responders). The parameters analyzed were parasitic load determined by q-PCR, hemogram, serum biochemistry and immune system-related gene expression signature. A mathematical model was applied to the analysis of these parameters to predict how efficient their response to therapy would be. Responder dogs restored hematological and biochemical parameters to the reference values and exhibited a Th1 cell activation profile with a linear tendency to reach mild clinical alteration stages. Differently, non-responders developed a mixed Th1/Th2 response and exhibited markers of liver and kidney injury. Erythrocyte counts and serum phosphorus were identified as predictive markers of therapeutic response at an early period of assessment of CanL. The results presented in this study are highly encouraging and may represent a new paradigm for future assistance to clinicians to interfere precociously in the therapeutic approach, with a more precise definition in the patient’s prognosis.
Publisher version
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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