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TitleIn silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome
Author(s)Cambeiro-Pérez, Noelia
Hidalgo-Cantabrana, Claudio
Moro-García, Marco Antonio
Blanco-Míguez, Aitor
Fdez-Riverola, Florentino
Riestra, Sabino
Lourenço, Anália
Alonso-Arias, Rebeca
Margolles, Abelardo
Martínez-Carballo, Elena
Sánchez, Borja
KeywordsExtracellular proteins
Innate immune system
Issue dateJul-2020
JournalJournal of Functional Foods
CitationCambeiro-Pérez, Noelia; Hidalgo-Cantabrana, Claudio; Moro-García, Marco Antonio; Blanco-Míguez, Aitor; Fdez-Riverola, Florentino; Riestra, Sabino; Lourenço, Anália; Alonso-Arias, Rebeca; Margolles, Abelardo; Martínez-Carballo, Elena; Sánchez, Borja, In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome. Journal of Functional Foods, 70(103969), 2020
Abstract(s)This work supports the massive presence of potential immunomodulatory peptides in the human gut metaproteome. These peptides were identified through the MAHMI database as potentially anti-inflammatory, and sixteen of them synthesized for characterize their mechanism of action. From them, peptide HM14 was encrypted in an extracellular protein produced by Bifidobacterium longum, a common member of the human microbiota, and displayed the highest anti-inflammatory capability. Molecular mechanism of action of HM14 pointed to a specific interaction between this immunomodulatory peptide and antigen presenting cells, which resulted in a higher formation of iTreg cells. Moreover, HM14 was effective in decreasing pro-inflammatory parameters in PBMCs isolated from a cohort of Crohns patients. Finally, non-targeted metabolomics confirmed the ability of HM14 to modulate the metabolic activity of PBMCs to fulfil its energy and biosynthetic requirements. Overall, our combined in silico/multiomics approach supports the human gut metaproteome as a source for immunomodulatory peptides.
DescriptionSupplementary data to this article can be found online at https://
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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