Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/64266

TitleHoney as a strategy to fight Candida tropicalis in mixed-biofilms with Pseudomonas aeruginosa
Author(s)Fernandes, L.
Oliveira, A.
Henriques, Mariana
Rodrigues, Maria Elisa Costa
KeywordsCandida tropicalis
Pseudomonas aeruginosa
biofilms
honey therapy
antifungal agents
Issue date2020
PublisherMDPI
JournalAntibiotics
CitationFernandes, L.; Oliveira, A.; Henriques, Mariana; Rodrigues, M. Elisa, Honey as a strategy to fight Candida tropicalis in mixed-biofilms with Pseudomonas aeruginosa. Antibiotics, 9(2), 43, 2020
Abstract(s)Fungal contaminations with Candida species are commonly responsible for several infections, especially when associated to bacteria. The therapeutic approach commonly used is being compromised due to microbial resistances of these microorganisms to antimicrobial agents, especially in biofilm. The use of honey as an antimicrobial agent has been emerging as a valuable solution and proving its potential in planktonic and in biofilm cells. This work aims to assess the effect of different honeys on biofilms of Candida tropicalis and Pseudomonas aeruginosa. The effect of Portuguese heather (PH) and manuka honeys on planktonic growth of Candida was initially evaluated by determination of the minimum inhibitory concentrations (MIC). Then, the same effect was evaluated in mixed biofilms, by colony-forming units numeration and fluorescence microscopy. The combinations of honey plus fluconazole and gentamicin were also tested. The results showed that the honeys tested enabled a great reduction of C. tropicalis, both in planktonic (12.5% and 25% of MIC for PH and manuka) and in biofilm. In polymicrobial biofilms, the use of PH and manuka honeys was revealed to be a promising choice and an alternative treatment, since they were able to reduce cells from both species. No synergistic effect was observed in antimicrobial combinations assays against polymicrobial biofilms.
TypeArticle
URIhttp://hdl.handle.net/1822/64266
DOI10.3390/antibiotics9020043
ISSN2079-6382
Publisher versionhttps://www.mdpi.com/journal/antibiotics
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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