Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/64213

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dc.contributor.authorFilipe, Diogopor
dc.contributor.authorFernandes, Helenapor
dc.contributor.authorCastro, Carolinapor
dc.contributor.authorPeres, Helenapor
dc.contributor.authorOliva-Teles, Airespor
dc.contributor.authorBelo, Isabelpor
dc.contributor.authorSalgado, José Manuelpor
dc.date.accessioned2020-03-04T09:08:24Z-
dc.date.available2020-03-04T09:08:24Z-
dc.date.issued2020-02-
dc.identifier.citationFilipe, Diogo; Fernandes, Helena; Castro, Carolina; Peres, Helena; Oliva-Teles, Aires; Belo, Isabel; Salgado, José Manuel, Improved lignocellulolytic enzyme production and antioxidant extraction using solid-state fermentation of olive pomace mixed with winery waste. Biofuels Bioproducts & Biorefining-Biofpr, 14(1), 78-91, 2020por
dc.identifier.issn1932-104Xpor
dc.identifier.urihttps://hdl.handle.net/1822/64213-
dc.description.abstractOlive pomace is characterized by its low nutritional value and high phenolic content, which hinders its direct use as animal feed, fertilizer, or as a substrate in bioprocesses such as solidstate fermentation (SSF). A possible strategy for bioprocessing olive pomace by SSF is the mixture of olive mill wastes with other wastes produced in the same region, such as winery wastes. This may improve the production of bioactive compounds like enzymes and antioxidant phenolics. A simplexcentroid design was used to evaluate the use of olive mill and winery wastes alone or in combination as a substrate for SSF with Aspergillus niger and Aspergillus ibericus. Synergistic effects of combinations of crude olive pomace (COP), exhausted olive pomace (EOP), vine trimming shoots (VTS), and exhausted grape marc (EGM) were observed in the production of xylanases, cellulases, glucosidases, and in the variation in total phenolics and antioxidant activity of SFF extracts. A multiple response optimization was carried out, leading to the following optimal mixture of substrates: for A. niger, 23% (w/w) COP, 30% EGM, 33% VTS, 14% EOP; for A. ibericus, 30% EGM, 36% VTS, 34% EOP. The scaleup to tray bioreactor with optimal substrate made it possible to achieve the maximum xylanase, cellulase, and glucosidase production of 189.1 ± 26.7, 56.3 ± 2.1 and 10.9 ± 0.8 U/g, respectively. The antioxidant activity of fermented wastes was also improved 2.2fold as compared with unfermented wastes. Thus, a combination of olive mill and winery wastes in SSF is a potential strategy to increase their value and to develop a circular strategy in these industries. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd.por
dc.description.sponsorshipJosé Manuel Salgado was supported by grant CEB/N2020 – INV/01/2016 from Project ‘BIOTECNORTE – Underpinning Biotechnology to foster the north of Portugal bioeconomy’ (NORTE-01-0145-FEDER-000004). This study was supported by SPO3 project, reference POCI-010145-FEDER-030377, funded by European Regional Development Fund (ERDF) and Portuguese Foundation for Science and Technology (FCT), by the InovFeed project, reference MAR-02.01.01- FEAMP-0111. This study was supported by the FCT under the scope of the strategic funding of UID/BIO/04469/2019 unit and by the BioTecNorte operation (NORTE-01-0145- FEDER-000004) funded by the ERDF under the scope of North 2020 – Northern Regional Operational Program. Helena Fernandes and Carolina Castro were supported by PhD grant SFRH/BD/131219/2017 and post-doctoral grant SFRH/BDP/114942/2016, funded by the Portuguese Foundation for Science and Technology (FCT), respectivelypor
dc.language.isoengpor
dc.publisherWiley-Blackwellpor
dc.relationUID/BIO/04469/2019por
dc.relationSFRH/BD/131219/2017por
dc.relationSFRH/BDP/114942/2016por
dc.rightsopenAccesspor
dc.subjectbiorefinerypor
dc.subjectolive mill wastespor
dc.subjectsolid-state fermentationpor
dc.subjectlignocellulolytic enzymespor
dc.titleImproved lignocellulolytic enzyme production and antioxidant extraction using solid-state fermentation of olive pomace mixed with winery wastepor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/journal/19321031por
dc.commentsCEB53362por
oaire.citationStartPage78por
oaire.citationEndPage91por
oaire.citationIssue1por
oaire.citationVolume14por
dc.date.updated2020-01-21T09:42:52Z-
dc.identifier.doi10.1002/bbb.2073por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersion-
dc.subject.wosScience & Technologypor
sdum.journalBiofuels, Bioproducts & Biorefiningpor
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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