Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/64154

TítuloPerillaldehyde 1,2-epoxide loaded SLN-tailored mAb: production, physicochemical characterization and in vitro cytotoxicity profile in MCF-7 cell lines
Autor(es)Souto, Eliana B.
Souto, Selma B.
Zielinska, Aleksandra
Durazzo, Alessandra
Lucarini, Massimo
Santini, Antonello
Horbánkczuk, Olaf K.
Atanasov, Atanas G.
Marques, Conrado
Andrade, Luciana N.
Silva, Amélia M.
Severino, Patricia
Palavras-chaveperillaldehyde 1,2-epoxide
Compritol ATO 888
cationic SLN
streptavidin adsorption
MCF-7 cells
perillaldehyde 1
2-epoxide
Data16-Fev-2020
EditoraMultidisciplinary Digital Publishing Institute (MDPI)
RevistaPharmaceutics
CitaçãoSouto, Eliana; Souto, Selma B.; Zielinska, Aleksandra; Durazzo, Alessandra; Lucarini, Massimo; Santini, Antonello; Horbánkczuk, Olaf K.; Atanasov, Atanas G.; Marques, Conrado; Andrade, Luciana N.; Silva, Amélia M.; Severino, Patricia, Perillaldehyde 1,2-epoxide loaded SLN-tailored mAb: production, physicochemical characterization and in vitro cytotoxicity profile in MCF-7 cell lines. Pharmaceutics, 12(2), 161, 2020.
Resumo(s)We have developed a new cationic solid lipid nanoparticle (SLN) formulation, composed of Compritol ATO 888, poloxamer 188 and cetyltrimethylammonium bromide (CTAB), to load perillaldehyde 1,2-epoxide, and surface-tailored with a monoclonal antibody for site-specific targeting of human epithelial growth receptor 2 (HER2). Perillaldehyde 1,2-epoxide-loaded cationic SLN (cPa-SLN), with a mean particle size (z-Ave) of 275.31 ± 4.78 nm and polydispersity index (PI) of 0.303 ± 0.081, were produced by high shear homogenization. An encapsulation efficiency of cPa-SLN above 80% was achieved. The release of perillaldehyde 1,2-epoxide from cationic SLN followed the Korsemeyer–Peppas kinetic model, which is typically seen in nanoparticle formulations. The lipid peroxidation of cPa-SLN was assessed by the capacity to produce thiobarbituric acid-reactive substances, while the antioxidant activity was determined by the capacity to scavenge the stable radical DPPH. The surface functionalization of cPa-SLN with the antibody was done via streptavidin-biotin interaction, monitoring z-Ave, PI and ZP of the obtained assembly (cPa-SLN-SAb), as well as its stability in phosphate buffer. The effect of plain cationic SLN (c-SLN, monoterpene free), cPa-SLN and cPa-SLN-SAb onto the MCF-7 cell lines was evaluated in a concentration range from 0.01 to 0.1 mg/mL, confirming that streptavidin adsorption onto cPa-SLN-SAb improved the cell viability in comparison to the cationic cPa-SLN.
TipoArtigo
URIhttps://hdl.handle.net/1822/64154
DOI10.3390/pharmaceutics12020161
ISSN1999-4923
e-ISSN1999-4923
Versão da editorahttps://www.mdpi.com/journal/pharmaceutics
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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