Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/64114

TítuloCollagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
Autor(es)Sousa, Rita Alexandra Oliveira
Martins, Eva
Carvalho, Duarte Nuno
Alves, Ana Luísa Silva
Oliveira, Ana Catarina Freitas Salazar
Duarte, Ana Rita C.
Silva, Tiago José Quinteiros Lopes Henriques
Reis, R. L.
Palavras-chaveCO2 acidified water
Cod skins
Fish collagen
Green extraction
Marine biomaterial
supercritical fluids
Marine biomaterials
CO acidified water 2
DataFev-2020
EditoraSpringer
RevistaJournal of Polymer Research
CitaçãoSousa R. O., Martins E., Carvalho D. N., Alves A. L., Oliveira C., Duarte A. R. C., Silva T. H., Reis R. L. Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare, Journal Of Polymer Research, Vol. 27, Issue 3, doi:10.1007/s10965-020-02048-x, 2020
Resumo(s)The extraction of collagen from fish skins is being proposed as strategy for valorization of marine origin by-products, being a sustainable alternative to mammal collagen. The method commonly uses solutions of organic acids, but new methodologies are arising, aiming to improve process yields and/or the properties of the resulting products. In this work, skins removed from salt brine Atlantic cod (Gadus morhua) were used to extract collagen, using water acidified with CO2, obtaining an extraction yield of 13.8% (w/w). Acidified water extracted collagen (AWC) presented a total content of proline-like amino acids of 151/1000 residues, with a degree of hydroxylation of 38%, and its SDS-PAGE profile is compatible with type I collagen. Moreover, FTIR, CD and XRD results suggest the presence of preserved triple helix, having a denaturation temperature of 32.3 °C as determined by micro-DSC. AWC exhibited a typical shear thinning behavior, interesting regarding their further processing, namely in jelly-like formulations. Additionally, the presence of AWC in MRC-5 human fibroblasts culture did not affect cell viability, demonstrating the non-cytotoxic behavior. Overall, the results support the efficiency of the proposed approach for collagen extraction and further enable the design of methodologies to address AWC use in biomedical or cosmetic context.
TipoArtigo
URIhttps://hdl.handle.net/1822/64114
DOI10.1007/s10965-020-02048-x
ISSN1022-9760
Versão da editorahttps://doi.org/10.1007/s10965-020-02048-x
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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