Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/63305

Full metadata record
DC FieldValueLanguage
dc.contributor.authorFaria-Oliveira, Fábiopor
dc.contributor.authorCarvalho, Joanapor
dc.contributor.authorBelmiro, Celso L Rpor
dc.contributor.authorMartinez-Gomariz, Montserratpor
dc.contributor.authorHernaez, Maria Luisapor
dc.contributor.authorPavão, Mauropor
dc.contributor.authorGil, Conchapor
dc.contributor.authorLucas, Cândidapor
dc.contributor.authorFerreira, Céliapor
dc.date.accessioned2020-01-20T12:33:16Z-
dc.date.available2020-01-20T12:33:16Z-
dc.date.issued2014-10-25-
dc.identifier.issn1471-2180por
dc.identifier.urihttp://hdl.handle.net/1822/63305-
dc.description.abstractIn a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support and protect bacterial and fungal multicellular aggregates, such as biofilms or colonies. However, the roles and composition of this microbial ECM are still poorly understood.por
dc.description.sponsorshipAuthors would like to acknowledge Joana Tulha for assistance on yeasts overlay photographs, and to Rui Armada for C. albicans ECM SDS-PAGE experiment. The proteomic analysis was carried out at the proteomics facility UCM-PCM, a member of the ProteoRed network. The polysaccharide analysis was performed at the Laboratory of Glycoconjugates Biochemistry and Cellular Biology, UFRJ, Brazil. Fabio Faria-Oliveira was supported by a PhD scholarship SFRH/BD/45368/2008 from FCT (Fundacao para a Ciencia e a Tecnologia). This work was funded by Marie Curie Initial Training Network GLYCOPHARM (PITN-GA-2012-317297), and by FCT/MEC through Portuguese funds (PIDDAC) - PEst-OE/BIA/UI4050/2014. The authors would also like to acknowledge Hugh S. Johnson for critical reading of the manuscript regarding English usage.por
dc.language.isoengpor
dc.publisherBMCpor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F45368%2F2008/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/135919/PTpor
dc.rightsopenAccesspor
dc.subjectCandida albicanspor
dc.subjectChemistry Techniques, Analyticalpor
dc.subjectExtracellular Matrixpor
dc.subjectFungal Proteinspor
dc.subjectGlycomicspor
dc.subjectPolysaccharidespor
dc.subjectProteomicspor
dc.subjectReproducibility of Resultspor
dc.subjectSaccharomyces cerevisiaepor
dc.titleMethodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomicspor
dc.typearticlepor
dc.peerreviewedyespor
oaire.citationIssue1por
oaire.citationVolume14por
dc.identifier.doi10.1186/s12866-014-0244-0por
dc.identifier.pmid25344425por
dc.subject.wosScience & Technologypor
sdum.journalBMC Microbiologypor
oaire.versionVoRpor
Appears in Collections:DBio - Artigos/Papers

Files in This Item:
File Description SizeFormat 
s12866-014-0244-0.pdf975,34 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID