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TitleRole of the DHH1 gene in the regulation of monocarboxylic acids transporters expression in Saccharomyces cerevisiae
Author(s)Mota, Sandra
Vieira, Neide
Barbosa, Sónia
Delaveau, Thierry
Torchet, Claire
Le Saux, Agnès
Garcia, Mathilde
Pereira, Ana
Lemoine, Sophie
Coulpier, Fanny
Darzacq, Xavier
Benard, Lionel
Casal, Margarida
Devaux, Frédéric
Paiva, Sandra
KeywordsAdaptation, Physiological
DEAD-box RNA Helicases
Gene Expression Profiling
Genome-Wide Association Study
Monocarboxylic Acid Transporters
RNA Stability
RNA, Messenger
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Gene Expression Regulation, Fungal
Issue date2014
PublisherPublic Library of Science (PLOS)
JournalPLoS ONE
Abstract(s)Previous experiments revealed that DHH1, a RNA helicase involved in the regulation of mRNA stability and translation, complemented the phenotype of a Saccharomyces cerevisiae mutant affected in the expression of genes coding for monocarboxylic-acids transporters, JEN1 and ADY2 (Paiva S, Althoff S, Casal M, Leao C. FEMS Microbiol Lett, 1999, 170:301-306). In wild type cells, JEN1 expression had been shown to be undetectable in the presence of glucose or formic acid, and induced in the presence of lactate. In this work, we show that JEN1 mRNA accumulates in a dhh1 mutant, when formic acid was used as sole carbon source. Dhh1 interacts with the decapping activator Dcp1 and with the deadenylase complex. This led to the hypothesis that JEN1 expression is post-transcriptionally regulated by Dhh1 in formic acid. Analyses of JEN1 mRNAs decay in wild-type and dhh1 mutant strains confirmed this hypothesis. In these conditions, the stabilized JEN1 mRNA was associated to polysomes but no Jen1 protein could be detected, either by measurable lactate carrier activity, Jen1-GFP fluorescence detection or western blots. These results revealed the complexity of the expression regulation of JEN1 in S. cerevisiae and evidenced the importance of DHH1 in this process. Additionally, microarray analyses of dhh1 mutant indicated that Dhh1 plays a large role in metabolic adaptation, suggesting that carbon source changes triggers a complex interplay between transcriptional and post-transcriptional effects.
AccessOpen access
Appears in Collections:DBio - Artigos/Papers

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