Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/62870

TitleEnthesis tissue engineering: biological requirements meet at the interface
Author(s)Calejo, I.
Costa-Almeida, R.
Reis, R. L.
Gomes, Manuela E.
Keywords2D vs 3D culture
Cell-based strategies
Gradient Biomaterials
growth factors
tendon-to-bone interface
Tissue engineering
2D versus 3D culture
Issue dateApr-2019
PublisherMary Ann Liebert
JournalTissue Engineering Part B: Reviews
CitationCalejo I., Costa-Almeida R., Reis R. L., Gomes M. E. Enthesis tissue engineering: biological requirements meet at the interface, Tissue Engineering Part B: Reviews, doi:10.1089/ten.TEB.2018.0383, 2019
Abstract(s)Tendon-to-bone interface (enthesis) exhibits a complex multiscale architectural and compositional organization maintained by a heterogeneous cellular environment. Orthopedic surgeons have been facing several challenges when treating tendon pullout or tear from the bony insertion due to unsatisfactory surgical outcomes and high retear rates. The limited understanding of enthesis hinders the development of new treatment options toward enhancing regeneration. Mimicking the natural tissue structure and composition is still a major challenge to be overcome. In this review, we critically assess current tendon-to-bone interface tissue engineering strategies through the use of biological, biochemical, or biophysical cues, which must be ultimately combined into sophisticated gradient systems. Cellular strategies are described, focusing on cell sources and cocultures to emulate a physiological heterotypic niche, as well as hypoxic environments, alongside with growth factor delivery and the use of platelet-rich hemoderivatives. Biomaterial design considerations are revisited, highlighting recent progresses in tendon-to-bone scaffolds. Mechanical loading is addressed to uncover prospective engineering advances. Finally, research challenges and translational aspects are considered. In summary, we highlight the importance of deeply investigating enthesis biology toward establishing foundational expertise and integrate cues from the native niche into novel biomaterial engineering, aiming at moving today's research advances into tomorrow's regenerative therapies.
TypeArticle
URIhttp://hdl.handle.net/1822/62870
DOI10.1089/ten.TEB.2018.0383
ISSN1937-3376
Publisher versionhttps://www.liebertpub.com/doi/abs/10.1089/ten.TEB.2018.0383
Peer-Reviewedyes
AccessEmbargoed access (1 Year)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
19836-reviewTEB_Enthesis tissue engineering- biological requirements meet at the interface.pdf
  Until 2020-05-01
1,55 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID