Please use this identifier to cite or link to this item:

TitleA molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis
Author(s)Becuwe, Michel
Vieira, Neide
Lara, David
Gomes-Rezende, Jéssica
Soares-Cunha, Carina
Casal, Margarida
Haguenauer-Tsapis, Rosine
Vincent, Olivier
Paiva, Sandra
Léon, Sébastien
Endosomal Sorting Complexes Required for Transport
Green Fluorescent Proteins
Membrane Proteins
Membrane Transport Proteins
Monocarboxylic Acid Transporters
Protein Processing, Post-Translational
Protein-Serine-Threonine Kinases
Saccharomyces cerevisiae Proteins
Ubiquitin-Protein Ligase Complexes
Signal Transduction
Issue date23-Jan-2012
PublisherRockefeller University Press
JournalJournal of Cell Biology
Abstract(s)Endocytosis regulates the plasma membrane protein landscape in response to environmental cues. In yeast, the endocytosis of transporters depends on their ubiquitylation by the Nedd4-like ubiquitin ligase Rsp5, but how extracellular signals trigger this ubiquitylation is unknown. Various carbon source transporters are known to be ubiquitylated and endocytosed when glucose-starved cells are exposed to glucose. We show that this required the conserved arrestin-related protein Rod1/Art4, which was activated in response to glucose addition. Indeed, Rod1 was a direct target of the glucose signaling pathway composed of the AMPK homologue Snf1 and the PP1 phosphatase Glc7/Reg1. Glucose promoted Rod1 dephosphorylation and its subsequent release from a phospho-dependent interaction with 14-3-3 proteins. Consequently, this allowed Rod1 ubiquitylation by Rsp5, which was a prerequisite for transporter endocytosis. This paper therefore demonstrates that the arrestin-related protein Rod1 relays glucose signaling to transporter endocytosis and provides the first molecular insights into the nutrient-induced activation of an arrestin-related protein through a switch in post-translational modifications.
Publisher version
AccessOpen access
Appears in Collections:DBio - Artigos/Papers

Files in This Item:
File Description SizeFormat 
jcb_201109113.pdf2,28 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID